Fujimori Shigeo, Washio Takanori, Higo Kenichi, Ohtomo Yasuhiro, Murakami Kazuo, Matsubara Kenichi, Kawai Jun, Carninci Piero, Hayashizaki Yoshihide, Kikuchi Shoshi, Tomita Masaru
Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0035, Japan.
FEBS Lett. 2003 Nov 6;554(1-2):17-22. doi: 10.1016/s0014-5793(03)01041-x.
A computer-based analysis was conducted to assess the characteristics of microsatellites in transcribed regions of rice and Arabidopsis. In addition, two mammals were simultaneously analyzed for a comparative analysis. Our analyses confirmed a novel plant-specific feature in which there is a gradient in microsatellite density along the direction of transcription. It was also confirmed that pyrimidine-rich microsatellites are found intensively near the transcription start sites, specifically in the two plants, but not in the mammals. Our results suggest that microsatellites located at high frequency in the 5'-flanking regions of plant genes can potentially act as factors in regulating gene expression.
进行了基于计算机的分析，以评估水稻和拟南芥转录区域中微卫星的特征。此外，同时分析了两种哺乳动物以进行比较分析。我们的分析证实了一种新的植物特异性特征，即沿转录方向微卫星密度存在梯度。还证实富含嘧啶的微卫星在转录起始位点附近密集存在，特别是在这两种植物中，但在哺乳动物中不存在。我们的结果表明，在植物基因5'侧翼区域高频出现的微卫星可能潜在地作为调节基因表达的因子。
