Corey Joseph M, Feldman Eva L
Department of Neurology, University of Michigan, Ann Arbor, MI, USA.
Exp Neurol. 2003 Nov;184 Suppl 1:S89-96. doi: 10.1016/s0014-4886(03)00392-3.
Extracellular matrix (ECM) proteins and cell-cell adhesion molecules (CAM) play important roles in neuronal development and differentiation. In the investigation of these roles, patterned substrates have proven to be a notably useful tool. Photolithographic and microprinting techniques can be used to make patterns of ECMs, CAMs, amino acids, and organofunctional groups for culturing neurons and other cell types. Experiments performed using these substrates have provided unique insights into the roles of cell-substratum adhesion, cell shape, and ECM composition on important cell functions, including survival, migration, neurite outgrowth, and development of polarity. Patterns may also be designed to localize cell bodies and confine their processes to predetermined areas of a substrate. Finally, the behavior of neurons on patterned substrates may prove helpful in the design of scaffoldings and nerve guides tailored for regeneration and repair of the nervous system.
细胞外基质（ECM）蛋白和细胞间粘附分子（CAM）在神经元发育和分化过程中发挥着重要作用。在对这些作用的研究中，图案化基质已被证明是一种非常有用的工具。光刻和微印刷技术可用于制作细胞外基质、细胞间粘附分子、氨基酸和有机官能团的图案，用于培养神经元和其他细胞类型。使用这些基质进行的实验为细胞与基质粘附、细胞形状和细胞外基质组成对重要细胞功能（包括存活、迁移、神经突生长和极性发育）的作用提供了独特的见解。图案也可以设计成定位细胞体并将其突起限制在基质的预定区域。最后，神经元在图案化基质上的行为可能有助于设计用于神经系统再生和修复的支架和神经导管。
