Naidu Kamatham A, Nasir Aejaz, Pinkas Haim, Kaiser Hans E, Brady Patric, Coppola Domenico
Baylor-Sammons Cancer Center, Dallas, Texas, USA.
In Vivo. 2003 Sep-Oct;17(5):479-82.
Glutathione-S-transferase (GST) isoenzymes are involved in the conjugation of glutathione to electrophilic carcinogens. Recent studies have shown increased levels and activities of GST in different tumors suggesting their role in carcinogen detoxification. This study compared GST activity levels and GST-pi protein expression in paired samples of colorectal cancer, adenoma and adjacent normal mucosa from a total of thirteen patients. GST was isolated from human colorectal specimens and assayed spectrophotometrically; Western immunoblot analysis was used to quantify GST-pi levels. GST activity was greater in both colorectal cancer and adenomas than in adjacent normal colonic tissue, although statistical significance was achieved only when comparing colorectal cancer to normal tissue. Based on these observations, we conclude that increased GST activity may be a useful marker of colonic neoplasia.
谷胱甘肽 - S - 转移酶（GST）同工酶参与谷胱甘肽与亲电子致癌物的结合反应。最近的研究表明，不同肿瘤中GST的水平和活性增加，提示其在致癌物解毒过程中的作用。本研究比较了来自13例患者的配对样本（包括结直肠癌、腺瘤及相邻正常黏膜）中GST的活性水平和GST - π蛋白表达。从人结肠标本中分离出GST并用分光光度法进行检测；采用Western免疫印迹分析法定量GST - π水平。结直肠癌和腺瘤中的GST活性均高于相邻正常结肠组织，不过只有在将结直肠癌与正常组织进行比较时才具有统计学意义。基于这些观察结果，我们得出结论：GST活性增加可能是结肠肿瘤形成的一个有用标志物。
