Morikubo Soichi, Takamura Yoshihiro, Kubo Eri, Tsuzuki Shosai, Akagi Yoshio
Department of Ophthalmology, Fukui Medical University, 23-3 Shimoaizuki, Matsuoka-machi, Yoshida-gun, Fukui 910-1193, Japan.
Nippon Ganka Gakkai Zasshi. 2003 Oct;107(10):565-70.
To evaluate the inhibitory effect of aldose reductase inhibitor (ARI) for the accumulation of sugar alcohol and the enhanced proliferation of lens epithelial cells of rats fed a galactose diet.
Sprague-Dawley rats were fed a diet containing 50% galactose with or without ARI (SNK-860). Histological changes in the lenses were observed by light microscopy, and the amount of galactitol accumulated in the lens epithelium was measured by liquid-gas chromatography. Immuno-histochemical staining of proliferating cell nuclear antigen (PCNA) in whole-mount preparations was performed to assay the proliferative ability of the lens epithelial cells.
The amount of galactitol in the lens epithelium and the number of PCNA positive cells in rats administered ARI were less than in rats fed the galactose diet. Multi-layered epithelium was observed in advanced cataract of rats fed the galactose diet, but not in the rats given ARI.
The administration of ARI can prevent cataractous changes and aberrant proliferation of lens epithelial cells.
评估醛糖还原酶抑制剂（ARI）对喂食半乳糖饮食的大鼠糖醇积累及晶状体上皮细胞增殖增强的抑制作用。
将斯普拉格-道利大鼠喂食含50%半乳糖的饮食，添加或不添加ARI（SNK-860）。通过光学显微镜观察晶状体的组织学变化，并用液-气相色谱法测量晶状体上皮中积累的半乳糖醇量。对整装制剂中的增殖细胞核抗原（PCNA）进行免疫组织化学染色，以检测晶状体上皮细胞的增殖能力。
给予ARI的大鼠晶状体上皮中的半乳糖醇量及PCNA阳性细胞数均少于喂食半乳糖饮食的大鼠。喂食半乳糖饮食的大鼠晚期白内障中观察到多层上皮，但给予ARI的大鼠中未观察到。
给予ARI可预防晶状体的白内障变化及上皮细胞的异常增殖。
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