通过胰管刷检检测胰腺癌中第12密码子的K-ras基因突变
Detection of K-ras gene mutation at codon 12 by pancreatic duct brushing for pancreatic cancer.
作者信息
Liu Feng, Li Zhao-Shen, Xu Guo-Ming, Sun Zhen-Xing, Zhou Guo-Xiong, Ren Xue-Xin, Tu Zhen-Xing, Gong Yan-Fang
机构信息
Department of Gastroenterology, Changhai Hospital, Second Military Medical Univercity, Shanghai 200433, China.
出版信息
Hepatobiliary Pancreat Dis Int. 2003 May;2(2):313-7.
OBJECTIVE
To assess the diagnostic value of endoscopic pancreatic duct brushing in detecting mutation of the K-ras gene at codon 12 in cytologic specimens from patients with pancreatic cancer.
METHODS
Thirty-five patients treated at Changhai Hospital, Shanghai between 1999 and 2001 were enrolled. Their cells obtained by pancreatic duct brushing during endoscopic retrograde cholangiopancreatography (ERCP) were suspended with phosphate buffer solution (PBS). DNA of the cells was extracted and mutation of the K-ras gene at codon 12 detected by means of PCR-SSCP.
RESULTS
The K-ras gene mutation rate of pancreatic cancer was 70%, which was higher than that of chronic pancreatitis (14%, P<0.05). K-ras gene mutation was not found in patients with pancreatic cystocarcinoma and duodenum carcinoma. As to the location of pancreatic cancer, no significant difference was observed between the head, the body and tail. The sensitivity, specificity, accuracy of pancreatic duct brushing in detecting pancreatic cancer was 70%, 94%, and 83%, respectively.
CONCLUSION
K-ras analysis of pancreatic brushing samples is helpful in the diagnosis of patients with early pancreatic cancer.
目的
评估内镜下胰管刷检在检测胰腺癌患者细胞学标本中第12密码子K-ras基因突变的诊断价值。
方法
纳入1999年至2001年期间在上海长海医院接受治疗的35例患者。通过内镜逆行胰胆管造影术（ERCP）期间胰管刷检获得的细胞，用磷酸盐缓冲液（PBS）悬浮。提取细胞DNA，并通过聚合酶链反应-单链构象多态性（PCR-SSCP）检测第12密码子K-ras基因突变。
结果
胰腺癌的K-ras基因突变率为70%，高于慢性胰腺炎（14%，P<0.05）。胰腺囊腺癌和十二指肠癌患者未发现K-ras基因突变。关于胰腺癌的位置，胰头、胰体和胰尾之间未观察到显著差异。胰管刷检检测胰腺癌的敏感性、特异性和准确性分别为70%、94%和83%。
结论
对胰腺刷检样本进行K-ras分析有助于早期胰腺癌患者的诊断。
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