评估IRIS 939 UDx流式显微镜作为尿路感染筛查系统的性能。
Evaluation of the IRIS 939 UDx flow microscope as a screening system for urinary tract infection.
作者信息
Hughes C, Roebuck M J
机构信息
Department of Medical Microbiology, Rotherham General Hospitals NHS Trust, Rotherham S60 2UD, UK.
出版信息
J Clin Pathol. 2003 Nov;56(11):844-9. doi: 10.1136/jcp.56.11.844.
AIMS
To evaluate the IRIS flow microscope for the examination of urine specimens and to compare its performance with that of manual methods; in addition, to assess its usefulness as a screening system for the detection of significant bacteriuria in a routine clinical laboratory.
METHODS
The IRIS system was compared with manual quantitative microscopy for the detection of formed elements and with a variety of culture methods to detect urinary tract infection.
RESULTS
The IRIS had a higher sensitivity for the detection of clinically significant formed elements than did manual methods, and using a combination of bacterial and white cell counts produced a sensitivity and specificity of 94.9% and 54.4%, respectively, for predicting positive significant growth, with a 98.5% predictive value for a negative result. Approximately 50% of specimens could be reported as negative on the day of receipt using the screening algorithm.
CONCLUSIONS
The sensitivity and specificity of the IRIS is equivalent to that of other automated screening systems and would remove the need for manual microscopy for most specimens. The system was easy to operate and is a useful addition to the methods currently available for the examination of urine specimens.
目的
评估用于尿液标本检查的IRIS流式显微镜，并将其性能与手工方法进行比较；此外，评估其作为常规临床实验室中检测显著菌尿的筛查系统的实用性。
方法
将IRIS系统与用于检测有形成分的手工定量显微镜检查以及多种检测尿路感染的培养方法进行比较。
结果
与手工方法相比，IRIS对检测具有临床意义的有形成分具有更高的灵敏度，并且结合细菌和白细胞计数预测阳性显著生长的灵敏度和特异度分别为94.9%和54.4%，阴性结果的预测值为98.5%。使用筛查算法，大约50%的标本在收到当天即可报告为阴性。
结论
IRIS的灵敏度和特异度与其他自动筛查系统相当，并且对于大多数标本无需进行手工显微镜检查。该系统易于操作，是目前可用的尿液标本检查方法的有益补充。
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