用于超灵敏检测N-酰基高丝氨酸内酯型群体感应分子的农杆菌生物测定菌株：检测华癸中生根瘤菌中的自诱导物
Agrobacterium bioassay strain for ultrasensitive detection of N-acylhomoserine lactone-type quorum-sensing molecules: detection of autoinducers in Mesorhizobium huakuii.
作者信息
Zhu Jun, Chai Yunrong, Zhong Zengtao, Li Shunpeng, Winans Stephen C
机构信息
Department of Microbiology, Cornell University, Ithaca, New York 14853, USA.
出版信息
Appl Environ Microbiol. 2003 Nov;69(11):6949-53. doi: 10.1128/AEM.69.11.6949-6953.2003.
Abstract
An ultrasensitive bioassay system for the detection of N-acylhomoserine lactones (AHLs) was constructed in Agrobacterium tumefaciens by using the T7 expression system to overproduce the AHL receptor TraR. This strain detected many diverse AHLs, some at extremely low concentrations. We used this strain to detect for the first time AHLs made by Mesorhizobium huakuii, which symbiotically fixes nitrogen in association with the legume Astragalus sinicus, a source of green manure throughout eastern Asia.
摘要
通过使用T7表达系统在根癌农杆菌中过量表达AHL受体TraR，构建了一种用于检测N-酰基高丝氨酸内酯（AHLs）的超灵敏生物测定系统。该菌株能检测到多种不同的AHLs，其中一些浓度极低。我们首次使用该菌株检测了华癸中生根瘤菌产生的AHLs，华癸中生根瘤菌与豆科植物紫云英共生固氮，紫云英是东亚各地绿肥的来源。
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