健康个体中甘露聚糖结合凝集素相关丝氨酸蛋白酶-2的水平。

Levels of mannan-binding lectin-associated serine protease-2 in healthy individuals.

作者信息

Møller-Kristensen Mette, Jensenius Jens Chr, Jensen Lisbeth, Thielens Nicole, Rossi Véronique, Arlaud Gerard, Thiel Steffen

机构信息

Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus C 8000, Denmark.

出版信息

J Immunol Methods. 2003 Nov;282(1-2):159-67. doi: 10.1016/j.jim.2003.08.012.

DOI:10.1016/j.jim.2003.08.012

PMID:14604549

Abstract

The lectin pathway is part of the innate immune system providing a first line of defence against infections. Mannan-binding lectin (MBL) and ficolins, in complex with MBL-associated serine proteases (MASPs), are capable of activating the complement system, thus mediating the destruction of infectious agents. MASP-2 cleaves C4 and C2 and is thus crucial for the activation of downstream complement components. We present an assay for quantifying total MASP-2 in plasma and serum samples. The assay is a sandwich type assay using a combination of two monoclonal anti-MASP-2 antibodies, one directed against the N-terminal part of MASP-2 and the other against its C-terminal part. Based on a population of Danish blood donors, the average MASP-2 concentration was estimated at 534 (S.D.+/-213) ng per ml of plasma. Characterization of the MASP-2 protein in serum showed high stability at 4 degrees C and at ambient temperature but a rapid decline at 37 degrees C. Gel permeation chromatography (GPC) indicated that all MASP-2 in serum is present in complexes with MBL and ficolins.

摘要

凝集素途径是固有免疫系统的一部分，为抵御感染提供第一道防线。甘露聚糖结合凝集素（MBL）和纤维胶凝蛋白与MBL相关丝氨酸蛋白酶（MASP）形成复合物，能够激活补体系统，从而介导对感染因子的破坏。MASP-2可裂解C4和C2，因此对下游补体成分的激活至关重要。我们提出了一种用于定量血浆和血清样本中总MASP-2的检测方法。该检测方法是一种夹心型检测方法，使用两种抗MASP-2单克隆抗体的组合，一种针对MASP-2的N端部分，另一种针对其C端部分。基于丹麦献血者群体，估计血浆中MASP-2的平均浓度为每毫升534（标准差±213）纳克。血清中MASP-2蛋白的特性表明，其在4℃和室温下具有高稳定性，但在37℃时迅速下降。凝胶渗透色谱法（GPC）表明，血清中的所有MASP-2都以与MBL和纤维胶凝蛋白的复合物形式存在。