Akalal David B G, Cummins Scott F, Painter Sherry D, Nagle Gregg T
Department of Anatomy and Neurosciences, Marine Biomedical Institute, University of Texas Medical Branch, Galveston, TX 77555, , USA.
Peptides. 2003 Aug;24(8):1117-22. doi: 10.1016/j.peptides.2003.06.001.
Mate attraction in Aplysia involves long-distance water-borne signaling via the secretion of the peptide pheromone attractin from the exocrine albumen gland during egg laying. Previous studies have shown that a second exocrine organ, the atrial gland, produces abundant egg-laying hormone (ELH) precursor-related peptides and mollusk-derived growth factor (MDGF), and crude extracts of the atrial gland are attractive in T-maze attraction assays. However, it is not known whether these peptides and proteins are secreted during egg laying. In this report, seawater eluates of freshly laid egg cordons were concentrated and fractionated by C18 RP-HPLC, and the resulting major peaks were examined by amino acid compositional analysis, microsequence analysis, and electrospray mass spectrometry. Concentrated egg cordon eluates were also examined by immunoblot analysis using anti-MDGF antisera as probe. The combined data demonstrated that the atrial gland of Aplysia californica does not secrete detectable levels of either ELH precursor-related peptides or MDGF during egg laying. Although the atrial gland is the last major exocrine organ to make contact with eggs before they are laid, the gland does not appear to secrete water-borne peptide pheromones during egg laying.
海兔的配偶吸引涉及产卵期间通过外分泌蛋白腺分泌肽类信息素吸引素来进行远距离水传播信号传递。先前的研究表明，另一个外分泌器官——心房腺，会产生大量与产卵激素（ELH）前体相关的肽和软体动物衍生生长因子（MDGF），并且心房腺的粗提物在T迷宫吸引试验中具有吸引力。然而，尚不清楚这些肽和蛋白质在产卵期间是否会分泌。在本报告中，对新产下的卵带的海水洗脱液进行浓缩，并通过C18反相高效液相色谱法进行分离，然后通过氨基酸组成分析、微序列分析和电喷雾质谱法对所得的主要峰进行检测。还使用抗MDGF抗血清作为探针，通过免疫印迹分析对浓缩的卵带洗脱液进行检测。综合数据表明，加利福尼亚海兔的心房腺在产卵期间不会分泌可检测水平的ELH前体相关肽或MDGF。尽管心房腺是卵子产下之前最后一个与卵子接触的主要外分泌器官，但该腺体在产卵期间似乎不会分泌水传播的肽类信息素。
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