槲皮素对血小板-内皮细胞黏附及其黏附分子表达的影响
[Effects of quercetin on platelet-endothelial cell adhesion and their expression of adhesion molecules].
作者信息
Liang Zhong-qin, Zhu Yi, Gu Zhen-lun, Lu Qun, Fan Pan-sheng
机构信息
Department of Pharmacology, Suzhou University Medical College, Suzhou, 215007 China.
出版信息
Yao Xue Xue Bao. 2003 Aug;38(8):574-7.
AIM
To observe the effect of quercetin (Que) on the adhesion of platelets to cultured endothelial cells and adhesion molecule expression by human umbilical vein endothelial cells (HUVEC) and platelets.
METHODS
[3H]-Adenine labeled platelets were incubated with HUVEC to investigate the effect of Que on adhesion of platelets to HUVEC. The number of platelets adhering to the HUVEC monolayer was determined by liquid scintillation spectroscopy. TNF-alpha induced HUVEC expression ICAM-1 and thrombin induced platelets expression of P-selectin were measured by flow cytometry.
RESULTS
Que (0.3-2.4 mumol.L-1) was shown to inhibit the increase of P-selectin expression of thrombin activated platelets. Pretreatment of HUVEC with tumor necrosis factor (TNF-alpha) significantly increased platelets adhesion to HUVEC and the expression ICAM-1. Que (0.6-2.4 mumol.L-1) inhibited this effect of TNF-alpha in a concentration-dependent manner.
CONCLUSION
Que can inhibit the adhesion of platelets to HUVEC and the expression of adhesion molecules (P-selectin and ICAM-1).
目的
观察槲皮素（Que）对血小板与培养的内皮细胞黏附以及人脐静脉内皮细胞（HUVEC）和血小板黏附分子表达的影响。
方法
将[³H] - 腺嘌呤标记的血小板与HUVEC共同孵育，以研究Que对血小板与HUVEC黏附的影响。通过液体闪烁光谱法测定黏附于HUVEC单层的血小板数量。采用流式细胞术检测肿瘤坏死因子-α（TNF-α）诱导的HUVEC中细胞间黏附分子-1（ICAM-1）的表达以及凝血酶诱导的血小板中P-选择素的表达。
结果
Que（0.3 - 2.4 μmol·L⁻¹）可抑制凝血酶激活的血小板中P-选择素表达的增加。用肿瘤坏死因子（TNF-α）预处理HUVEC可显著增加血小板与HUVEC的黏附以及ICAM-1的表达。Que（0.6 - 2.4 μmol·L⁻¹）以浓度依赖的方式抑制TNF-α的这种作用。
结论
Que可抑制血小板与HUVEC的黏附以及黏附分子（P-选择素和ICAM-1）的表达。
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