Tanapongpipat Sutipa, Luxananil Plearnpis, Promdonkoy Boonhiang, Chewawiwat Namchai, Audtho Mongkon, Panyim Sakol
BIOTEC Central Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Phaholyothin Rd, Klong 1, Klongluang, Pathumthani 12120, Thailand.
FEMS Microbiol Lett. 2003 Nov 21;228(2):259-63. doi: 10.1016/S0378-1097(03)00780-8.
A recombinant plasmid harboring cry4A, cry4B and cry11A from Bacillus thuringiensis subsp. israelensis and binary toxin genes from Bacillus sphaericus has been constructed. The three cry genes were placed under the control of the cry4B promoter whereas the binary toxin gene was controlled by its native promoter. The expression of toxins in Escherichia coli harboring the resulting plasmid, p4BDA-5142, was investigated. Cry4B expression was highest compared to other toxins. Although the level of toxin expression was low compared with E. coli expressing single toxins, the recombinant E. coli strain harboring p4BDA-5142 exhibited broad range mosquito-larvicidal activity against all Aedes, Culex and Anopheles larvae. This work has shown that the development of the recombinant plasmid can be used to broaden the host range spectrum of the appropriate bacterial host for mosquito control.
已构建了一种重组质粒，其携带来自苏云金芽孢杆菌以色列亚种的cry4A、cry4B和cry11A以及来自球形芽孢杆菌的二元毒素基因。三个cry基因置于cry4B启动子的控制之下，而二元毒素基因则由其天然启动子控制。对携带所得质粒p4BDA - 5142的大肠杆菌中毒素的表达进行了研究。与其他毒素相比，Cry4B的表达最高。尽管与表达单一毒素的大肠杆菌相比，毒素表达水平较低，但携带p4BDA - 5142的重组大肠杆菌菌株对所有伊蚊、库蚊和按蚊幼虫均表现出广泛的杀蚊幼虫活性。这项工作表明，重组质粒的开发可用于扩大用于蚊虫控制的合适细菌宿主的宿主范围谱。
