人丝氨酸脱水酶的结晶及初步晶体学分析
Crystallization and preliminary crystallographic analysis of human serine dehydratase.
作者信息
Sun Lei, Li Xuemei, Dong Yanjie, Yang Maojun, Liu Yiwei, Han Xueqing, Zhang Xianen, Pang Hai, Rao Zihe
机构信息
MOE Protein Science Laboratory and Laboratory of Structural Biology, Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China.
出版信息
Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2297-9. doi: 10.1107/s0907444903020110. Epub 2003 Nov 27.
L-Serine dehydratase (SDH) catalyzes the pyridoxal phosphate (PLP) dependent deamination of L-serine to yield pyruvate. Recombinant human serine dehydratase was crystallized by the hanging-drop vapour-diffusion method. Crystals were grown at 291 K using (NH4)(2)SO4 as precipitant. Diffraction data were obtained to a resolution of 2.5 A from a single frozen crystal using Cu Kalpha radiation. The crystal belongs to space group I422, with unit-cell parameters a = 157.4, b = 157.4, c = 59.2 A, alpha = beta = gamma = 90 degrees. The asymmetric unit contains one molecule and has a solvent content of about 46%.
L-丝氨酸脱水酶（SDH）催化依赖磷酸吡哆醛（PLP）的L-丝氨酸脱氨反应生成丙酮酸。重组人丝氨酸脱水酶通过悬滴气相扩散法结晶。使用硫酸铵（(NH4)2SO4）作为沉淀剂，在291 K下生长晶体。使用铜Kα辐射从单个冷冻晶体获得了分辨率为2.5 Å的衍射数据。该晶体属于空间群I422，晶胞参数为a = 157.4，b = 157.4，c = 59.2 Å，α = β = γ = 90°。不对称单元包含一个分子，溶剂含量约为46%。
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