Zhou Wei, Dong Yi, Zhao Yan, Tang Fu-lin
Department of Rheumatology and Clinical Immunology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003 Oct;25(5):603-7.
To investigate whether interferon-inducible protein 10 (IP-10) is involved in the inflammatory process of the labial gland of patients with Sjogren's Syndrome (SS).
Forty-nine patients performed labial gland biopsy, the number of lymphocytes in the biopsy tissues was calculated and the IP-10 was detected by the methods as following: 39 biopsied labial tissues were examined by RT-PCR, among them, 21 were from primary SS, 5 from secondary SS and 13 from other diseases. With RT-PCR, the IP-10 and beta-actin were co-amplified with specific primers. The gel-fractioned and ethidium bromide amplification products were then analyzed by densitometry. The expression of IP-10 was semi-quantificated by IP-10/beta-actin ratio. Twenty-one samples were examined by immunohistochemistry with specific goat anti-IP-10 antibody, 10 of them from primary SS, 3 from secondary SS, 8 from other diseases. 11 out of 21 samples were examined by both RT-PCR and immunohistochemistry.
The expression of IP-10 mRNA was significantly up-regulated in labial glands of patients with SS compared with other diseases (IP-10/beta-actin ratio was 0.329 +/- 0.157 vs 0.099 +/- 0.059, P < 0.01). The number of lymphocyte infiltration foci in labial glands of patients with SS correlated to the IP-10/beta-actin ratio (r = 0.657, P < 0.05). Ductal epithelial cells and some of the infiltrating lymphocytes were stained by anti-IP-10 antibody by immunohistochemistry in 8 of the primary SS (8/10), all of the secondary SS (3/3) and one with primary biliary sclerosis (1/8). The expression of IP-10 protein detected by immunohistochemistry was consistent with that of mRNA detected by RT-PCR.
IP-10 is abnormally highly expressed in the labial glands of patients with SS and positively relates to the lymphocyte infiltration. It thus suggests chemokine IP-10 may be one of the important molecules attracting the lymphocytes to the minor salivary glands to form the lymphocytic foci of Sjogren's Syndrome.
研究干扰素诱导蛋白10（IP-10）是否参与干燥综合征（SS）患者唇腺的炎症过程。
49例患者进行唇腺活检，计算活检组织中的淋巴细胞数量，并采用以下方法检测IP-10：39例唇腺活检组织采用RT-PCR检测，其中21例来自原发性SS，5例来自继发性SS，13例来自其他疾病。采用RT-PCR，用特异性引物共扩增IP-10和β-肌动蛋白。然后对凝胶分离并经溴化乙锭染色的扩增产物进行光密度分析。通过IP-10/β-肌动蛋白比值对IP-10的表达进行半定量。21例样本采用特异性山羊抗IP-10抗体进行免疫组织化学检测，其中10例来自原发性SS，3例来自继发性SS，8例来自其他疾病。21例样本中的11例同时采用RT-PCR和免疫组织化学检测。
与其他疾病相比，SS患者唇腺中IP-10 mRNA的表达显著上调（IP-10/β-肌动蛋白比值为0.329±0.157 vs 0.099±0.059，P<0.01）。SS患者唇腺中淋巴细胞浸润灶的数量与IP-10/β-肌动蛋白比值相关（r = 0.657，P<0.05）。免疫组织化学检测显示，8例原发性SS（8/10）、所有继发性SS（3/3）和1例原发性胆汁性硬化患者（1/8）的导管上皮细胞和部分浸润淋巴细胞被抗IP-10抗体染色。免疫组织化学检测到的IP-10蛋白表达与RT-PCR检测到的mRNA表达一致。
IP-10在SS患者唇腺中异常高表达，且与淋巴细胞浸润呈正相关。因此提示趋化因子IP-10可能是吸引淋巴细胞至小涎腺形成干燥综合征淋巴细胞灶的重要分子之一。
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