Park W S, Chae J S, Jung K C, Choi W J, Kook M-C, Bae Y
Department of Pathology, Kangwon National University College of Medicine, Chuncheon, Korea.
Tissue Antigens. 2004 Jan;63(1):46-53. doi: 10.1111/j.1399-0039.2004.00151.x.
A monoclonal antibody against human CD43 has been developed and designated as K06. Its reactivity in the lymphoid organs was different from that of known anti-CD43 monoclonal antibodies suggesting that this may recognize a novel epitope of human CD43 molecule. The CD43 epitope detected by anti-K06 monoclonal antibody was highly expressed in cortical thymocytes, platelets, and myeloid cells of normal peripheral blood, and its reactivity was comparable to that of known anti-CD43 monoclonal antibodies. However, the density of this epitope was lower in the medullary thymocytes. Biochemical studies indicated that anti-K06 monoclonal antibody could recognize glycosylated moiety of CD43 antigen. The expression profile of anti-K06 monoclonal antibody in several cell lines was somewhat different from that of known anti-CD43 antibodies. In addition, CD43 ligation through the K06 epitope appeared to induce apoptosis in human leukemic cell line, Molt-4. We therefore assume that K06 epitope of human CD43 might have some role in T-cell development.
一种针对人CD43的单克隆抗体已被研制出来并命名为K06。其在淋巴器官中的反应性与已知抗CD43单克隆抗体不同，这表明它可能识别了人CD43分子的一个新表位。抗K06单克隆抗体检测到的CD43表位在正常外周血的皮质胸腺细胞、血小板和髓样细胞中高度表达，其反应性与已知抗CD43单克隆抗体相当。然而，该表位在髓质胸腺细胞中的密度较低。生化研究表明，抗K06单克隆抗体能够识别CD43抗原的糖基化部分。抗K06单克隆抗体在几种细胞系中的表达谱与已知抗CD43抗体略有不同。此外，通过K06表位连接CD43似乎可诱导人白血病细胞系Molt-4发生凋亡。因此，我们推测人CD43的K06表位可能在T细胞发育中发挥某种作用。
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