用展示表面锚定的人乳头瘤病毒16型E7癌蛋白的活乳酸乳球菌对小鼠进行免疫接种。
Mice immunization with live lactococci displaying a surface anchored HPV-16 E7 oncoprotein.
作者信息
Cortes-Perez Naima G, Bermúdez-Humarán Luis G, Le Loir Yves, Rodriguez-Padilla Cristina, Gruss Alexandra, Saucedo-Cárdenas Odila, Langella Philippe, Montes-de-Oca-Luna Roberto
机构信息
Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, N.L. 66450, Mexico.
出版信息
FEMS Microbiol Lett. 2003 Dec 5;229(1):37-42. doi: 10.1016/S0378-1097(03)00778-X.
E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-positive bacterium Lactococcus lactis. An efficient cell wall anchoring of E7 was obtained. Intranasal administration of these recombinant lactococci in mice induced an HPV-16 E7-specific immune response. This is the first report of E7 cell wall anchoring in L. lactis and represents one more step towards the use of live food-grade bacteria to fight against CxCa.
人乳头瘤病毒16型（HPV - 16）的E7癌蛋白在宫颈癌（CxCa）中持续产生，是治疗性疫苗设计的良好候选对象。在本研究中，使用乳链菌肽控制表达系统将E7蛋白展示在食品级革兰氏阳性菌乳酸乳球菌的细胞表面。实现了E7在细胞壁上的有效锚定。对小鼠进行这些重组乳球菌的鼻内给药诱导了HPV - 16 E7特异性免疫反应。这是E7在乳酸乳球菌细胞壁上锚定的首次报道，代表了朝着利用活的食品级细菌对抗宫颈癌迈出的又一步。
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