Reynolds John N J, Wickens Jeffery R
Department of Anatomy and Structural Biology, School of Medical Sciences, and the Neuroscience Research Centre, University of Otago, PO Box 913, Dunedin, New Zealand.
J Neurosci Methods. 2003 Dec 30;131(1-2):111-9. doi: 10.1016/j.jneumeth.2003.08.003.
This paper describes the circuitry and construction of a novel electronic threshold discriminator, and details its specific application to in vivo intracellular recording. The discriminator reliably triggers electrophysiological recording at pre-selectable membrane potentials in neuronal systems that exhibit membrane potential oscillations. It has been used successfully whilst recording from spiny projection neurons of the striatum to measure membrane properties and trigger electrical stimulation within either of two discrete membrane potential "states". The device works by comparing the analogue membrane potential waveform with a user-defined threshold membrane potential, and outputs a logic signal to flag the occurrence of a threshold-crossing event. This signal is used to trigger the commencement of episodic recording and the application of current injection or electrical stimulation at a consistent membrane potential. Thus, the discriminator acts as a functional clamp to isolate evoked responses from endogenous fluctuations in membrane potential. The unit uses cheap and easily available components and can be constructed with the minimum of electronics experience. It could be adapted to isolate discrete events within any oscillatory system.
本文描述了一种新型电子阈值鉴别器的电路和构造，并详细介绍了其在体内细胞内记录中的具体应用。该鉴别器能在呈现膜电位振荡的神经元系统中，以预先选定的膜电位可靠地触发电生理记录。在从纹状体的棘状投射神经元进行记录时，它已成功用于测量膜特性，并在两种离散的膜电位“状态”中的任何一种状态下触发电刺激。该装置通过将模拟膜电位波形与用户定义的阈值膜电位进行比较来工作，并输出一个逻辑信号以标记阈值穿越事件的发生。这个信号用于触发间歇性记录的开始，并在一致的膜电位下进行电流注入或电刺激。因此，鉴别器起到了功能钳的作用，将诱发反应与膜电位的内源性波动隔离开来。该装置使用廉价且易于获得的组件，只需最少的电子学经验就能构建。它可以被改装用于隔离任何振荡系统内的离散事件。
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