苏云金芽孢杆菌昆虫亚种LBIT-113的质粒pUIBI-1的测序与特性分析
Sequencing and characterization of plasmid pUIBI-1 from Bacillus thuringiensis serovar entomocidus LBIT-113.
作者信息
López-Meza Joel E, Barboza-Corona J Eleazar, Del Rincón-Castro M Cristina, Ibarra Jorge E
机构信息
Centro Multidisciplinario de Estudios en Biotecnología-FMVZ, Univ. Michoacana de San Nicolás de Hidalgo, Apdo. Postal 53, Administración Chapultepec, 58262 Morelia, Michoacán, México.
出版信息
Curr Microbiol. 2003 Nov;47(5):395-9. doi: 10.1007/s00284-003-4041-5.
Plasmid pUIBI-1 from Bacillus thuringiensis svr. entomocidus was sequenced and its replication mechanism analyzed. Sequence analysis revealed that pUIBI-1 contains 4671 bp and a 32% GC content. Plasmid pUIBI-1 also includes at least seven putative open reading frames (ORFs) encoding for proteins ranging from 5 to 50 kDa. ORF-1 encodes for a putative 16-kDa Rep protein, which lacks homology with proteins of similar function. ORF2 encodes for a protein of 50 kDa and shows homology with Mob proteins of plasmids pLUB1000 from Lactobacillus hilgardii (32.2%) and pGI2 from B. thuringiensis (33.7%). Detection of single-stranded DNA (ssDNA) intermediates indicated that pUIBI-1 replicates by the rolling-circle replication mechanism, as demonstrated by S1 treatment and Southern hybridization under non-denaturing conditions.
对来自苏云金芽孢杆菌昆虫亚种的质粒pUIBI - 1进行了测序，并分析了其复制机制。序列分析表明，pUIBI - 1含有4671个碱基对，GC含量为32%。质粒pUIBI - 1还包含至少七个推定的开放阅读框（ORF），编码5至50 kDa的蛋白质。ORF - 1编码一种推定的16 kDa Rep蛋白，该蛋白与功能相似的蛋白质缺乏同源性。ORF2编码一种50 kDa的蛋白质，与来自希氏乳杆菌的质粒pLUB1000（32.2%）和苏云金芽孢杆菌的pGI2（33.7%）的Mob蛋白具有同源性。单链DNA（ssDNA）中间体的检测表明，pUIBI - 1通过滚环复制机制进行复制，这在非变性条件下的S1处理和Southern杂交中得到了证明。
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