玉米中ZmTIP2-3的特性研究，ZmTIP2-3是一种具有水通道蛋白活性的根特异性液泡膜内在蛋白。

Characterization in maize of ZmTIP2-3, a root-specific tonoplast intrinsic protein exhibiting aquaporin activity.

作者信息

Lopez Félicie, Bousser Agnès, Sissoëff Igor, Hoarau Jackson, Mahé Aline

机构信息

Institut de Biotechnologie des Plantes, Bât. 630, CNRS/UMR 8618, Université Paris-Sud, F-91405 Orsay Cedex, France.

出版信息

J Exp Bot. 2004 Feb;55(396):539-41. doi: 10.1093/jxb/052. Epub 2003 Dec 12.

DOI:10.1093/jxb/052

PMID:14673023

Abstract

The characterization of the tonoplast intrinsic protein ZmTIP2-3 cDNA isolated from maize roots is reported. ZmTIP2-3 belongs to the TIP2 group according to the present nomenclature. The aquaporin function of ZmTIP2-3 protein was demonstrated using expression in X. laevis oocytes. Northern blot analyses revealed that ZmTIP2-3 was specifically expressed in roots. Salt and water stresses induced the accumulation of ZmTIP2-3 transcripts. By contrast, no effect of ABA was observed. An oscillation of ZmTIP2-3 transcript amount during the day-night cycle was observed with some typical features of genes regulated by a circadian mechanism.

摘要

本文报道了从玉米根中分离得到的液泡膜内在蛋白ZmTIP2-3 cDNA的特性。根据目前的命名法，ZmTIP2-3属于TIP2组。通过在非洲爪蟾卵母细胞中表达，证明了ZmTIP2-3蛋白的水通道蛋白功能。Northern杂交分析表明，ZmTIP2-3在根中特异性表达。盐胁迫和水分胁迫诱导了ZmTIP2-3转录本的积累。相比之下，未观察到脱落酸（ABA）的影响。在昼夜周期中观察到ZmTIP2-3转录本量的振荡，具有一些受昼夜节律机制调控的基因的典型特征。