Reinsmoen N L, Kersey J H, Yunis E J
J Natl Cancer Inst. 1978 Mar;60(3):537-43. doi: 10.1093/jnci/60.3.537.
Mixed lymphocyte culture (MLC) studies of families with several leukemia patients, all potential bone marrow transplant recipients, demonstrated that cells from acute myelogenous leukemia patients (5 of 5) and acute undifferentiated leukemia patients (1 of 4) in relapse stimulated autologous lymphocytes as well as lymphocytes from siblings known to be identical at the major histocompatibility linkage group. In the patients studied, the blast transformation induced by leukemia cells was not detectable when the patient was in remission. Stimulation by leukemia cells also elicited increased responses of the lymphocytes from normal haploidentical siblings, parents, and unrelated individuals as compared to stimulation by normal allogeneic cells or leukemia cells of patients with leukemia in remission. The primed lymphocyte test (PLT) was used successfully to establish HLA-D identity of the leukemia patients and their respective HLA-identical siblings, despite high percentages of circulatory blasts. Utilizing lymphocytes from normal siblings primed against the leukocytes from an HLA-identical sibling with leukemia, we also presented results of PLT's which suggested that the stimulation induced by leukemia cells in MLC was produced by leukemia-associated antigens.
对几个白血病患者家庭（所有患者均为潜在的骨髓移植受者）进行的混合淋巴细胞培养（MLC）研究表明，复发期的急性髓性白血病患者（5例中的5例）和急性未分化白血病患者（4例中的1例）的细胞不仅能刺激自身淋巴细胞，还能刺激已知在主要组织相容性连锁群上相同的同胞淋巴细胞。在所研究的患者中，当患者处于缓解期时，白血病细胞诱导的母细胞转化无法检测到。与正常同种异体细胞或处于缓解期的白血病患者的白血病细胞刺激相比，白血病细胞刺激还引起了正常半相合同胞、父母和无关个体的淋巴细胞反应增强。尽管循环母细胞比例很高，但致敏淋巴细胞试验（PLT）仍成功用于确定白血病患者及其各自的HLA相同同胞的HLA-D同一性。利用针对患有白血病的HLA相同同胞的白细胞致敏的正常同胞的淋巴细胞，我们还展示了PLT的结果，这些结果表明MLC中白血病细胞诱导的刺激是由白血病相关抗原产生的。
