一种与GM3（NeuGc）神经节苷脂相关的抗独特型单克隆抗体的产生与鉴定

Generation and characterization of an anti-idiotype monoclonal antibody related to GM3(NeuGc) ganglioside.

作者信息

Rodríguez Mabel, Llanes Leticia, Pérez Alexis, Pérez Rolando, Vázquez Ana María

机构信息

Department of Antibody Engineering, Center of Molecular Immunology, Havana, Cuba.

出版信息

Hybrid Hybridomics. 2003 Oct;22(5):307-14. doi: 10.1089/153685903322538836.

DOI:10.1089/153685903322538836

PMID:14678648

Abstract

The 14F7 monoclonal antibody (MAb), IgG1 isotype, which reacts specifically to GM3(NeuGc) ganglioside induced a specific IgG anti-idiotypic antibody (Ab2) response in syngeneic mice when it was administered coupled with KLH and in the presence of Freund's adjuvant. Spleen cells from these mice were used in somatic-cell hybridization experiments using the murine myeloma cell line P3-X63-Ag8 653 as fusion partner. An IgG1 Ab2 MAb was selected. This Ab2 MAb, called 4G9, was able to block the binding of 14F7 MAb to GM3(NeuGc) ganglioside and developed a strong IgG anti-anti-idiotypic antibody (Ab3) response, when injected into syngeneic mice. These Ab3 antibodies were characterized to bear 14F7 MAb idiotopes, but did not have the same specificity as 14F7 MAb. In the other hand, a very specific anti-NeuGc-containing ganglioside response was generated in chickens immunized with this Ab2 MAb, thus behaving, in this species as an "internal image" antibody.

摘要

14F7单克隆抗体（MAb），IgG1同种型，它与GM3（NeuGc）神经节苷脂特异性反应，当与钥孔血蓝蛋白（KLH）偶联并在弗氏佐剂存在的情况下给药时，在同基因小鼠中诱导了特异性IgG抗独特型抗体（Ab2）反应。来自这些小鼠的脾细胞用于体细胞杂交实验，使用鼠骨髓瘤细胞系P3-X63-Ag8 653作为融合伙伴。筛选出一种IgG1 Ab2 MAb。这种称为4G9的Ab2 MAb能够阻断14F7 MAb与GM3（NeuGc）神经节苷脂的结合，并且当注射到同基因小鼠中时会产生强烈的IgG抗抗独特型抗体（Ab3）反应。这些Ab3抗体被鉴定为带有14F7 MAb的独特型表位，但不具有与14F7 MAb相同的特异性。另一方面，在用这种Ab2 MAb免疫的鸡中产生了非常特异性的抗含NeuGc神经节苷脂反应，因此在该物种中表现为“内影像”抗体。