In vitro protein refolding by chromatographic procedures.
作者信息
Li Ming, Su Zhi-Guo, Janson Jan-Christer
机构信息
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, PR China.
出版信息
Protein Expr Purif. 2004 Jan;33(1):1-10. doi: 10.1016/j.pep.2003.08.023.
PMID:14680955
Abstract
In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results.
摘要
Zotero 插件