嗜热栖热菌红素还原蛋白的W3Y单突变体：飞行时间中子初步研究
W3Y single mutant of rubredoxin from Pyrococcus furiosus: a preliminary time-of-flight neutron study.
作者信息
Li Xinmin, Langan Paul, Bau Robert, Tsyba Irina, Jenney Francis E, Adams Michael W W, Schoenborn Benno P
机构信息
Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
出版信息
Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):200-2. doi: 10.1107/s0907444903024041. Epub 2003 Dec 18.
Rubredoxin from the hyperthermophilic archaeon Pyrococcus furiosus maintains its native structure at high temperatures (373 K). In order to investigate the role of hydrogen bonding, hydration and chain dynamics in this thermostability, wavelength-resolved Laue neutron diffraction data have been collected from the W3Y single mutant (Trp3-->Tyr3) on the spallation neutron protein crystallography station (PCS) at Los Alamos Neutron Science Center. Data were measured at room temperature from nine crystal settings, each of approximately 12 h duration. The total data-measurement period was less than 5 d from a single crystal that had undergone H(2)O/D(2)O exchange. The nominal resolution of the data is 2.1 A.
来自嗜热古菌激烈火球菌的红素氧还蛋白在高温（373 K）下维持其天然结构。为了研究氢键、水合作用和链动力学在这种热稳定性中的作用，已在洛斯阿拉莫斯中子科学中心的散裂中子蛋白质晶体学站（PCS）收集了来自W3Y单突变体（Trp3→Tyr3）的波长分辨劳厄中子衍射数据。在室温下从九个晶体设置测量数据，每个设置持续约12小时。从经过H₂O/D₂O交换的单个晶体获得的总数据测量期不到5天。数据的标称分辨率为2.1 Å。
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