血管内皮生长因子（VEGF）的所有三种受体均在B细胞慢性淋巴细胞白血病（CLL）细胞上表达。

All three receptors for vascular endothelial growth factor (VEGF) are expressed on B-chronic lymphocytic leukemia (CLL) cells.

作者信息

Bairey O, Boycov O, Kaganovsky E, Zimra Y, Shaklai M, Rabizadeh E

机构信息

Institute of Hematology and the Felsenstein Medical Research Center, Rabin Medical Center, Beilinson Campus Petah Tikva 49100, Israel.

出版信息

Leuk Res. 2004 Mar;28(3):243-8. doi: 10.1016/s0145-2126(03)00256-x.

DOI:10.1016/s0145-2126(03)00256-x

PMID:14687619

Abstract

B-chronic lymphocytic leukemia (B-CLL) cells have a long survival owing to an alteration in the normal pathways of apoptosis. CLL cells have been found to produce and secrete vascular endothelial growth factor (VEGF). In addition to its major role in angiogenesis, VEGF affects cell survival by interfering with apoptosis. The aim of the present study was to investigate the expression of the VEGF receptors VEGFR-1, VEGFR-2, and VEGFR-3 on B-CLL cells, singly and combined. B-CLL cells were isolated from peripheral blood drawn from patients with CLL. Total VEGF receptor, examined in 13 samples by flow cytometry was present in all cases with mean CD19+/VEGF+ expression of 76% (range 52-92%). Specific receptor expression, examined in 27 samples by immunocytochemical methods, was positive for VEGFR-1 in all 27 patients and for VEGFR-2 and VEGFR-3 in 26 (96%). These findings suggest that the VEGF transduction pathway may be very active in CLL cells, and both its paracrine and autocrine pathways may contribute to their enhanced survival.

摘要

B 细胞慢性淋巴细胞白血病（B-CLL）细胞由于正常凋亡途径的改变而具有较长的生存期。已发现 CLL 细胞能产生并分泌血管内皮生长因子（VEGF）。VEGF 除了在血管生成中起主要作用外，还通过干扰凋亡来影响细胞存活。本研究的目的是单独及联合研究 VEGF 受体 VEGFR-1、VEGFR-2 和 VEGFR-3 在 B-CLL 细胞上的表达。从 CLL 患者抽取的外周血中分离出 B-CLL 细胞。通过流式细胞术在 13 个样本中检测到的总 VEGF 受体在所有病例中均存在，平均 CD19+/VEGF+表达为 76%（范围 52%-92%）。通过免疫细胞化学方法在 27 个样本中检测到的特异性受体表达，在所有 27 例患者中 VEGFR-1 呈阳性，26 例（96%）患者中 VEGFR-2 和 VEGFR-3 呈阳性。这些发现表明 VEGF 转导途径在 CLL 细胞中可能非常活跃，其旁分泌和自分泌途径都可能有助于其生存期延长。