[中国维吾尔族和彝族人群中δ-氨基乙酰丙酸脱水酶多态性与铅中毒遗传易感性]

[The polymorphism of delta-aminolevulinic acid dehydratase and genetic susceptibility to lead toxicity in Uighur and Yi population in China].

作者信息

Lu Jing, Lu Xinfang, Cui Tao, Xie Guangyun, Wu Rongguo, Zhang Chen, He Xiwen

机构信息

Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Bejing 100050, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2002 Aug;20(4):277-81.

PMID:14694653

Abstract

OBJECTIVE

To investigate the polymorphism of delta-aminolevulinic acid dehydratase(ALAD) and the genetic susceptibility to lead toxicity in Uighur and Yi population in China.

METHODS

The ALAD genotypes were determined by PCR and MspI restriction fragment length polymorphism techniques in 214 Uighur individuals from Xinjiang autonomous region and 144 Yi individuals from Yunnan province. The correlation between the polymorphism of ALAD and blood lead levels, and the factors affecting the latter were explored.

RESULTS

The frequencies of the allele ALAD1 and ALAD2 in Uighur are 0.91 and 0.09; and in Yi are 0.98 and 0.02 respectively. In Uighur the average blood lead level was (76 +/- 4) microgram/L, and 25.7% individuals with blood lead level > or = 100 micrograms/L. In Yi the average blood lead level was (50 +/- 16) microgram/L, and 6.3% individuals with blood lead level > or = 100 micrograms/L. However, no statistic correlation between the distribution of ALAD alleles and the blood lead level was found in both populations.

CONCLUSION

The genetic susceptibility of ALAD polymorphism to lead toxicity may exhibit in a lead dose-dependent manner.

摘要

目的

研究中国维吾尔族和彝族人群中δ-氨基乙酰丙酸脱水酶（ALAD）的多态性及其对铅中毒的遗传易感性。

方法

采用聚合酶链反应（PCR）和MspI限制性片段长度多态性技术，对来自新疆自治区的214名维吾尔族个体和来自云南省的144名彝族个体进行ALAD基因分型。探讨ALAD多态性与血铅水平的相关性以及影响血铅水平的因素。

结果

维吾尔族中ALAD1和ALAD2等位基因频率分别为0.91和0.09；彝族中分别为0.98和0.02。维吾尔族人群平均血铅水平为（76±4）μg/L，血铅水平≥100μg/L的个体占25.7%。彝族人群平均血铅水平为（50±16）μg/L，血铅水平≥100μg/L的个体占6.3%。然而，在这两个人群中均未发现ALAD等位基因分布与血铅水平之间存在统计学相关性。