Kawata Kiyoshi, Takeyoshi Izumi, Iwanami Kotaro, Sunose Yutaka, Tsutsumi Hirofumi, Ohwada Susumu, Matsumoto Koshi, Morishita Yasuo
Second Department of Surgery, Gunma University School of Medicine, 3-39-15 Showa-Machi, Maebashi, Gunma 371-8511, Japan.
Hepatogastroenterology. 2003 Nov-Dec;50(54):1970-4.
BACKGROUND/AIMS: The purpose of this study was to investigate the effects of a selective COX-2 inhibitor, FK3311, on warm ischemia-reperfusion injury of the canine small intestine.
Ten adult mongrel dogs were used. FK (1 mg/kg) was administered intravenously 15 minutes prior to ischemia and 15 minutes prior to reperfusion in the FK group (n = 5), and only an inert vehicle was injected in the control group (n = 5). The superior mesenteric artery and vein were clamped closed for 2 hours and then unclamped for 12 hours of reperfusion. Arterial and intramucosal pH were measured, and samples were taken for histological examination at 1, 3, 6, and 12 hours after the start of reperfusion. Serum thromboxane B2 and 6-keto-prostaglandin F1 alpha (stable metabolites of TxA2 and PGI2) were measured 30 minutes after reperfusion began.
Arterial and intramucosal pH changes were significantly (p < 0.05) smaller in the FK group than in the control group. Histological ischemia-reperfusion injury was significantly (p < 0.05) more severe in the control group than in the FK group. Serum thromboxane B2 and 6-keto-prostaglandin F1 alpha levels were significantly (p < 0.05) lower in the FK group compared to the control group.
FK protects the small bowel from ischemia-reperfusion injury by suppression of prostanoid production.
背景/目的：本研究旨在探讨选择性环氧化酶-2抑制剂FK3311对犬小肠热缺血再灌注损伤的影响。
选用10只成年杂种犬。FK组（n = 5）在缺血前15分钟和再灌注前15分钟静脉注射FK（1 mg/kg），对照组（n = 5）仅注射惰性赋形剂。肠系膜上动脉和静脉夹闭2小时，然后松开进行12小时再灌注。在再灌注开始后1、3、6和12小时测量动脉和黏膜内pH值，并取样本进行组织学检查。再灌注开始30分钟后测量血清血栓素B2和6-酮-前列腺素F1α（血栓素A2和前列环素I2的稳定代谢产物）。
FK组动脉和黏膜内pH值变化显著（p < 0.05）小于对照组。对照组组织学缺血再灌注损伤显著（p < 0.05）比FK组更严重。与对照组相比，FK组血清血栓素B2和6-酮-前列腺素F1α水平显著（p < 0.05）降低。
FK通过抑制前列腺素生成保护小肠免受缺血再灌注损伤。
