Li Pei-yuan, Lin Ju-sheng, Feng Zuo-hua, Zhang Hui, Zhou He-jun, Zhang Jin-yan
Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhonghua Gan Zang Bing Za Zhi. 2003 Dec;11(12):716-8.
To construct and express secretive endostatin eukaryotic plasmid for treatment of hepatoma.
Mouse Igk signal peptide sequence was synthesized and cloned into pcDNA3.1 with endostatin gene. The supernant of BHK-21 transfected with recombinant was used to culture ECV304. The proliferation of latter was evaluated by MTT assay. H22 was inoculated intramusclely, then naked DNA of endostatin plasmid was injected into the inoculation site. Tumors were dissected and weighted after treatments. All data was analyzed by SPSS10.0.
The supernant of BHK-21 transfected with recombinant can inhibit the proliferation of ECV304 by 29.2%. Tumor weight lighter after injected with naked pSecES (1.34 g+/-0.96g) compared with naked pcDNA3.1 (2.70g+/-0.82g) and saline (3.73g+/-1.41g).
The endostatin eukaryotic plasmid was constructed and it can be used for gene therapy on hepatoma.
构建并表达用于治疗肝癌的分泌型内皮抑素真核质粒。
合成小鼠Igk信号肽序列，并将其与内皮抑素基因一起克隆到pcDNA3.1中。用重组体转染的BHK-21细胞的上清液培养ECV304。通过MTT法评估后者的增殖情况。将H22肌肉接种，然后将内皮抑素质粒的裸DNA注射到接种部位。处理后解剖肿瘤并称重。所有数据用SPSS10.0进行分析。
用重组体转染的BHK-21细胞的上清液可抑制ECV304的增殖达29.2%。与注射裸pcDNA3.1（2.70g±0.82g）和生理盐水（3.73g±1.41g）相比，注射裸pSecES后肿瘤重量更轻（1.34g±0.96g）。
构建了内皮抑素真核质粒，可用于肝癌的基因治疗。
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