Barbosa Maria J, Wijffels René H
Food and Bioprocess Engineering Group, Wageningen University, PO Box 8129, 6700 EV Wageningen, The Netherlands.
Biotechnol Bioeng. 2004 Jan 5;85(1):78-85. doi: 10.1002/bit.10862.
In the present work we identified and quantified the effect of hydrodynamic stress on two different microalgae strains, Dunaliella tertiolecta and D. salina, cultivated in bench-scale bubble columns. The cell death rate constant increased with increasing gas-entrance velocity at the sparger. Dunaliella salina was slightly more sensitive than D. tertiolecta. The critical gas-entrance velocities were approximately 50 and 30 m s(-1) for D. tertiolecta and D. salina, respectively. The effects of gas-flow rate, culture height, and nozzle diameter on the death rate constant were also studied. From these results it was concluded that bubble rising and bubble bursting are not responsible for cell death. Regarding nozzle diameter, small nozzles were more detrimental to cells. The bubble formation at the sparger was found to be the main event leading to cell death.
在本研究中，我们确定并量化了水力应力对在实验室规模鼓泡塔中培养的两种不同微藻菌株——三角褐指藻（Dunaliella tertiolecta）和盐生杜氏藻（D. salina）的影响。细胞死亡率常数随着分布器处气体入口速度的增加而升高。盐生杜氏藻比三角褐指藻稍敏感。三角褐指藻和盐生杜氏藻的临界气体入口速度分别约为50和30 m s⁻¹。还研究了气体流速、培养高度和喷嘴直径对死亡率常数的影响。从这些结果可以得出结论，气泡上升和气泡破裂不是细胞死亡的原因。关于喷嘴直径，小喷嘴对细胞更有害。发现分布器处的气泡形成是导致细胞死亡的主要因素。
