Montes H, Salmen S, Dolfo W, Sotolongo Asmiria, Petrosino Pierina, Donis J, Berrueta Lisbeth
Centro de Atención Médico Odontológico, Universidad de Los Andes, Camoula, Venezuela.
Acta Gastroenterol Latinoam. 2003;33(2):73-6.
The aim of our study was to develop a rapid diagnostic urease test to demonstrate the presence of Helicobacter pylori in the Endoscopy room.
200 consecutive patients referred to gastroscopy for different indications, were included in this study. One antral biopsy sample was obtained to be immersed in our test. The same sample was used for histological evaluation, considered to be the gold standard method for diagnose of Helicobacter pylori infection.
135 patients (67.5%) were found positives and 65 patients (32.5%) were negatives in our test. 128 patients (64%) showed Helicobacter pylori on histological examination. Our test showed a sensitivity of 91%, specificity of 88.1%, and positive and negative predictive values of 95% and 80% respectively. A remarkable correlation between density of Helicobacter pylori and reading time was also observed, where a high density of the bacteria reduced the reaction time in this liquid test. Furthermore, an overall accuracy of 90% was shown, which is comparable with other available commercial tests.
LUT is easy to handle, cost effective and fast, with a high positive predictive value.
我们研究的目的是开发一种快速诊断脲酶试验，以在内镜检查室检测幽门螺杆菌的存在。
本研究纳入了200例因不同指征接受胃镜检查的连续患者。获取一份胃窦活检样本并浸入我们的试验中。同一样本用于组织学评估，组织学评估被认为是诊断幽门螺杆菌感染的金标准方法。
在我们的试验中，135例患者（67.5%）呈阳性，65例患者（32.5%）呈阴性。128例患者（64%）在组织学检查中显示有幽门螺杆菌。我们的试验显示敏感性为91%，特异性为88.1%，阳性预测值和阴性预测值分别为95%和80%。还观察到幽门螺杆菌密度与读取时间之间存在显著相关性，即细菌密度高会缩短这种液体试验中的反应时间。此外，显示总体准确率为90%，这与其他现有的商业试验相当。
LUT易于操作、成本效益高且快速，具有较高的阳性预测值。
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