Aspen Stephen, Savage Harry M
Division of Vector-Borne Infectious Disease, Centers for Disease Control and Prevention, PO Box 2087, Fort Collins, CO 80522, USA.
J Am Mosq Control Assoc. 2003 Dec;19(4):323-8.
Nucleotide sequence differences in the acetylcholinesterase gene Ace.2 were used to develop an assay to distinguish among North American mosquitoes of the Culex pipiens complex. Taxon-specific polymerase chain reaction primers based on sequence differences within intron 2 of Ace.2 distinguish among the sibling species Cx. pipiens Linneaus and Cx. quinquefasciatus Say and their F1 hybrids. This assay may be used to confirm the species composition of mosquito pools, identify individual specimens collected in arbovirus surveillance programs and other mosquito studies, and define zones of hybridization.
乙酰胆碱酯酶基因Ace.2中的核苷酸序列差异被用于开发一种检测方法，以区分北美尖音库蚊复合组的蚊子。基于Ace.2内含子2内序列差异的分类群特异性聚合酶链反应引物可区分同胞物种尖音库蚊指名亚种（Cx. pipiens Linneaus）和致倦库蚊（Cx. quinquefasciatus Say）及其F1杂种。该检测方法可用于确认蚊虫样本的物种组成，鉴定在虫媒病毒监测项目和其他蚊虫研究中采集的个体样本，并确定杂交区域。
