来自马萨诸塞州科德角楠塔基特岛和韦尔弗利特的鹿蜱（肩突硬蜱）体内的细胞内共生菌及其他细菌。
Intracellular symbionts and other bacteria associated with deer ticks (Ixodes scapularis) from Nantucket and Wellfleet, Cape Cod, Massachusetts.
作者信息
Benson Micah J, Gawronski Jeffrey D, Eveleigh Douglas E, Benson David R
机构信息
Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269, USA.
出版信息
Appl Environ Microbiol. 2004 Jan;70(1):616-20. doi: 10.1128/AEM.70.1.616-620.2004.
Abstract
The diversity of bacteria associated with the deer tick (Ixodes scapularis) was assessed using PCR amplification, cloning, and sequencing of 16S rRNA genes originating from seven ticks collected from Nantucket Island and Wellfleet, Cape Cod, Mass. The majority of sequences obtained originated from gram-negative proteobacteria. Four intracellular bacteria were detected including strains of Ehrlichia, Rickettsia, and Wolbachia and an organism related to intracellular insect symbionts from the Cytophaga-Flavobacterium-Bacteroides group. Several strains of members of the Sphingomonadaceae were also detected in all but one tick. The results provide a view of the diversity of bacteria associated with I. scapularis ticks in the field.
摘要
利用聚合酶链反应（PCR）扩增、克隆及对16S rRNA基因进行测序的方法，对与肩突硬蜱（Ixodes scapularis）相关的细菌多样性进行了评估。这些16S rRNA基因源自从马萨诸塞州科德角楠塔基特岛和韦尔弗利特采集的7只蜱虫。获得的大多数序列源自革兰氏阴性变形菌。检测到四种细胞内细菌，包括埃立克体、立克次氏体和沃尔巴克氏体的菌株，以及一种与噬细胞-黄杆菌-拟杆菌群的细胞内昆虫共生体相关的生物体。除一只蜱虫外，在其他所有蜱虫中均检测到了鞘脂单胞菌科的几个菌株。研究结果展现了野外环境中与肩突硬蜱相关的细菌多样性情况。
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