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[利用ISSR-PCR分析普通啤酒花(Humulus lupulus L.)品种的分子遗传多态性]

[Molecular-genetic polymorphisms of cultivars of common hops (Humulus lupulus L.) using ISSR-PCR analysis].

作者信息

Danilova T V, Danilov S S, Karlov G I

机构信息

Timiryazev Agricultural Academy, Moscow, 127550 Russia.

出版信息

Genetika. 2003 Nov;39(11):1484-9.

Abstract

Genetic diversity among 26 Russian and European cultivars of the common hop (Humulus lupulus L.) was studied using the ISSR-PCR technique. Twenty-one primers used provided amplification of 183 DNA fragments, 106 of which (57.9%) were found to be polymorphic. The ISSR markers, specific for certain cultivars were revealed. Based on the coefficient of dissimilarity values, cluster analysis was performed and a dendrogram was constructed, on which most of the hop cultivars formed two clusters according to their origin. Advantages of the ISSR-PCR analysis in selective studies aimed at the classification and identification of common hop cultivars are discussed.

摘要

利用ISSR-PCR技术研究了26个俄罗斯和欧洲普通啤酒花(Humulus lupulus L.)品种的遗传多样性。所使用的21个引物扩增出183个DNA片段,其中106个(57.9%)具有多态性。揭示了某些品种特有的ISSR标记。基于差异系数值进行聚类分析并构建了树形图,在该图上,大多数啤酒花品种根据其起源形成了两个聚类。讨论了ISSR-PCR分析在普通啤酒花品种分类和鉴定的选择性研究中的优势。

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