Danilova T V, Danilov S S, Karlov G I
Timiryazev Agricultural Academy, Moscow, 127550 Russia.
Genetika. 2003 Nov;39(11):1484-9.
Genetic diversity among 26 Russian and European cultivars of the common hop (Humulus lupulus L.) was studied using the ISSR-PCR technique. Twenty-one primers used provided amplification of 183 DNA fragments, 106 of which (57.9%) were found to be polymorphic. The ISSR markers, specific for certain cultivars were revealed. Based on the coefficient of dissimilarity values, cluster analysis was performed and a dendrogram was constructed, on which most of the hop cultivars formed two clusters according to their origin. Advantages of the ISSR-PCR analysis in selective studies aimed at the classification and identification of common hop cultivars are discussed.
利用ISSR-PCR技术研究了26个俄罗斯和欧洲普通啤酒花(Humulus lupulus L.)品种的遗传多样性。所使用的21个引物扩增出183个DNA片段,其中106个(57.9%)具有多态性。揭示了某些品种特有的ISSR标记。基于差异系数值进行聚类分析并构建了树形图,在该图上,大多数啤酒花品种根据其起源形成了两个聚类。讨论了ISSR-PCR分析在普通啤酒花品种分类和鉴定的选择性研究中的优势。