Shapovalova N I, Ivanov L Iu, Matvienko N I
Bioorg Khim. 1992 Sep;18(9):1186-9.
A site-specific endonuclease activity was found in extract of Bacillus stearothermophilus M6 isolated from molasses. The functionally pure enzyme designated as BstM6I was obtained by consecutive chromatographies on blue sepharose, hydroxyapatite, and heparin-sepharose. The endonuclease recognizes the nucleotide sequence CC decreases WGG in double-stranded DNA and cleaves it as indicated by the arrow to give one-nucleotide 5'-protruding ends. Consequently, the site-specific endonuclease BstM6I is an isoshizomer of BstNI.
在从糖蜜中分离出的嗜热脂肪芽孢杆菌M6的提取物中发现了一种位点特异性内切核酸酶活性。通过在蓝色琼脂糖、羟基磷灰石和肝素琼脂糖上连续色谱法获得了功能纯的酶，命名为BstM6I。该内切核酸酶识别双链DNA中的核苷酸序列CC↓WGG，并按箭头所示进行切割，产生单核苷酸5'突出末端。因此，位点特异性内切核酸酶BstM6I是BstNI的同裂酶。
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