海绵细胞培养？一种海绵细胞的分子鉴定方法。

Sponge-cell culture? A molecular identification method for sponge cells.

作者信息

Sipkema Detmer, Heilig Hans G H J, Akkermans Antoon D L, Osinga Ronald, Tramper Johannes, Wijffels René H

机构信息

Wageningen University, Food and Bioprocess Engineering Group, PO Box 8129, 6700 EV Wageningen, The Netherlands.

出版信息

Mar Biotechnol (NY). 2003 Sep-Oct;5(5):443-9. doi: 10.1007/s10126-002-0090-1.

DOI:10.1007/s10126-002-0090-1

PMID:14730427

Abstract

Dissociated sponge cells are easily confused with unicellular organisms. This has been an obstacle in the development of sponge-cell lines. We developed a molecular detection method to identify cells of the sponge Dysidea avara in dissociated cell cultures. The 18S ribosomal RNA gene from a Dysidea avara specimen was sequenced and compared to eukaryotic 18S rDNA sequences picked up from a proliferating cell culture that originated from a dissociated Dysidea avara specimen. Our method proved unambiguously that this was not a sponge-cell culture. Therefore, it provides a valuable tool for further research on sponge-cell cultures.

摘要

解离的海绵细胞很容易与单细胞生物混淆。这一直是海绵细胞系发展的一个障碍。我们开发了一种分子检测方法，以在解离的细胞培养物中鉴定海绵阿氏皮海绵（Dysidea avara）的细胞。对一个阿氏皮海绵标本的18S核糖体RNA基因进行了测序，并与从源自解离的阿氏皮海绵标本的增殖细胞培养物中获取的真核18S rDNA序列进行了比较。我们的方法明确证明这不是海绵细胞培养物。因此，它为海绵细胞培养的进一步研究提供了一个有价值的工具。