Lagroye I, Hook G J, Wettring B A, Baty J D, Moros E G, Straube W L, Roti Roti J L
PIOM/EPHE Bioelectromagnetics Laboratory, ENSCPB, 16 Avenue Pey-Berland, 33607 Pessac, France.
Radiat Res. 2004 Feb;161(2):201-14. doi: 10.1667/rr3122.
In vitro experiments were performed to determine whether 2450 MHz microwave radiation induces alkali-labile DNA damage and/or DNA-protein or DNA-DNA crosslinks in C3H 10T(1/2) cells. After a 2-h exposure to either 2450 MHz continuous-wave (CW) microwaves at an SAR of 1.9 W/kg or 1 mM cisplatinum (CDDP, a positive control for DNA crosslinks), C3H 10T(1/2) cells were irradiated with 4 Gy of gamma rays ((137)Cs). Immediately after gamma irradiation, the single-cell gel electrophoresis assay was performed to detect DNA damage. For each exposure condition, one set of samples was treated with proteinase K (1 mg/ml) to remove any possible DNA-protein crosslinks. To measure DNA-protein crosslinks independent of DNA-DNA crosslinks, we quantified the proteins that were recovered with DNA after microwave exposure, using CDDP and gamma irradiation, positive controls for DNA-protein crosslinks. Ionizing radiation (4 Gy) induced significant DNA damage. However, no DNA damage could be detected after exposure to 2450 MHz CW microwaves alone. The crosslinking agent CDDP significantly reduced both the comet length and the normalized comet moment in C3H 10T(1/2) cells irradiated with 4 Gy gamma rays. In contrast, 2450 MHz microwaves did not impede the DNA migration induced by gamma rays. When control cells were treated with proteinase K, both parameters increased in the absence of any DNA damage. However, no additional effect of proteinase K was seen in samples exposed to 2450 MHz microwaves or in samples treated with the combination of microwaves and radiation. On the other hand, proteinase K treatment was ineffective in restoring any migration of the DNA in cells pretreated with CDDP and irradiated with gamma rays. When DNA-protein crosslinks were specifically measured, we found no evidence for the induction of DNA-protein crosslinks or changes in amount of the protein associated with DNA by 2450 MHz CW microwave exposure. Thus 2-h exposures to 1.9 W/ kg of 2450 MHz CW microwaves did not induce measurable alkali-labile DNA damage or DNA-DNA or DNA-protein crosslinks.
进行体外实验以确定2450兆赫微波辐射是否会在C3H 10T（1/2）细胞中诱导碱不稳定DNA损伤和/或DNA - 蛋白质或DNA - DNA交联。在以1.9瓦/千克的比吸收率暴露于2450兆赫连续波（CW）微波2小时或1毫摩尔顺铂（CDDP，DNA交联的阳性对照）后，用4戈瑞的γ射线（（137）Cs）照射C3H 10T（1/2）细胞。在γ射线照射后立即进行单细胞凝胶电泳分析以检测DNA损伤。对于每种暴露条件，一组样品用蛋白酶K（1毫克/毫升）处理以去除任何可能的DNA - 蛋白质交联。为了独立于DNA - DNA交联测量DNA - 蛋白质交联，我们使用CDDP和γ射线照射（DNA - 蛋白质交联的阳性对照）对微波暴露后与DNA一起回收的蛋白质进行定量。电离辐射（4戈瑞）诱导了显著的DNA损伤。然而，单独暴露于2450兆赫CW微波后未检测到DNA损伤。交联剂CDDP显著降低了用4戈瑞γ射线照射的C3H 10T（1/2）细胞中的彗星长度和归一化彗星矩。相比之下，2450兆赫微波并未阻碍γ射线诱导的DNA迁移。当对照细胞用蛋白酶K处理时，在没有任何DNA损伤的情况下，两个参数均增加。然而，在暴露于2450兆赫微波的样品或用微波和辐射组合处理的样品中未观察到蛋白酶K的额外作用。另一方面，蛋白酶K处理在恢复用CDDP预处理并用γ射线照射的细胞中DNA的任何迁移方面无效。当特异性测量DNA - 蛋白质交联时，我们没有发现2450兆赫CW微波暴露诱导DNA - 蛋白质交联或与DNA相关的蛋白质数量变化的证据。因此，以1.9瓦/千克暴露于2450兆赫CW微波2小时不会诱导可测量的碱不稳定DNA损伤或DNA - DNA或DNA - 蛋白质交联。
Mutat Res. 2000-11-20
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