通过蛋白质磷酸化控制SV40 DNA复制：细胞DNA复制的一种模型？

Control of SV40 DNA replication by protein phosphorylation: a model for cellular DNA replication?

作者信息

Fanning E

机构信息

Institute for Biochemistry, Karlstrasse 23, 80333 Munich, Germany.

出版信息

Trends Cell Biol. 1994 Jul;4(7):250-5. doi: 10.1016/0962-8924(94)90123-6.

DOI:10.1016/0962-8924(94)90123-6

PMID:14731665

Abstract

SV40 DNA replication has been studied extensively as a model for eukaryotic DNA replication. The initiation of SV40 DNA replication depends on certain cellular enzymes and on a multifunctional viral phosphoprotein, T antigen, whose activity is controlled positively and negatively by its phosphorylation state. Several cellular protein kinases and phosphatases that act on T antigen have now been identified. The recent elucidation of the step in initiation that is sensitive to T antigen's phosphorylation state raises the question of whether initiation of cellular DNA replication may utilize a similar regulatory mechanism.

摘要

SV40 DNA复制作为真核生物DNA复制的模型已被广泛研究。SV40 DNA复制的起始取决于某些细胞酶和一种多功能病毒磷蛋白T抗原，其活性受磷酸化状态的正向和负向控制。现在已经鉴定出几种作用于T抗原的细胞蛋白激酶和磷酸酶。最近对起始过程中对T抗原磷酸化状态敏感步骤的阐明，引发了细胞DNA复制起始是否可能利用类似调节机制的问题。