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玉蜀黍黑粉菌REC1基因对质粒-染色体重组的影响。

The influence of the Ustilago maydis REC1 gene on plasmid-chromosome recombination.

作者信息

Banks G R, Kanuga N, Ealing J, Spanos A, Holden D W

机构信息

Genetics Division, National Institute for Medical Research, Mill Hill, London, UK.

出版信息

Curr Genet. 1992 Dec;22(6):483-9. doi: 10.1007/BF00326414.

Abstract

The REC1 gene of U. maydis has an important but ill-defined role in DNA recombination and repair. We have examined its role in plasmid-chromosome recombination. Plasmid DNA was linearized at various locations with respect to the cloned U. maydis PYR3 gene and introduced into cells by transformation. Chromosomal integration and repair by an homologous cross-over with plasmid containing a double-strand break or gap in the PYR3 gene was markedly reduced in the absence of the REC1 gene product. Homologous replacement of the chromosomal pyr3-1 allele by a single copy of wild-type sequences from plasmid cut outside PYR3 was not found in the absence of the REC1 product. Instead, novel transformants generated in its absence suggests that ligation plays a role in their generation.

摘要

玉米黑粉菌的REC1基因在DNA重组和修复中具有重要但尚不明确的作用。我们研究了它在质粒-染色体重组中的作用。相对于克隆的玉米黑粉菌PYR3基因,质粒DNA在不同位置被线性化,并通过转化导入细胞。在缺乏REC1基因产物的情况下,与含有PYR3基因双链断裂或缺口的质粒进行同源交叉导致的染色体整合和修复显著减少。在缺乏REC1产物时,未发现质粒在PYR3外侧切割产生的单拷贝野生型序列对染色体pyr3-1等位基因的同源替换。相反,在缺乏REC1时产生的新型转化体表明连接在其产生过程中发挥了作用。

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