在滋养层细胞中，NEUROD1在体外作为NEUROD2的上游调节因子起作用。

NEUROD1 acts in vitro as an upstream regulator of NEUROD2 in trophoblast cells.

作者信息

Westerman Bart A, Chhatta Aniska, Poutsma Ankie, van Vegchel Thijs, Oudejans Cees B M

机构信息

Molecular Biology Laboratory, Department of Clinical Chemistry, VU University Medical Center, de Boelelaan 1117, 1081 HV Amsterdam, The Netherlands.

出版信息

Biochim Biophys Acta. 2004 Jan 5;1676(1):96-103. doi: 10.1016/j.bbaexp.2003.10.002.

DOI:10.1016/j.bbaexp.2003.10.002

PMID:14732494

Abstract

The basic helix-loop-helix (bHLH) transcription factors NEUROD1, NEUROD2 and ATH2 are expressed during first trimester human placental development. We determined the transactivation potential of each of these factors in trophoblasts by measuring changes in the endogenous gene activity using absolute quantification by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) after transient transfection. In these assays, NEUROD1 was found to transiently transactivate NEUROD2 in trophoblast cells. Promotor truncation assays, using luciferase constructs, showed the presence of two domains in the NEUROD2 promotor, which showed increased activity after NeuroD1 transfection. Each of these NeuroD1-responsive domains contains an E-box sequence. The NEUROD2 transactivation data fit with the spatial expression pattern of NEUROD1 and NEUROD2, since they are expressed in endovascular trophoblasts. This expression pattern, as well as the present transactivation results, might suggest the presence of a NEUROD differentiation cascade during first trimester human placental development.

摘要

碱性螺旋-环-螺旋（bHLH）转录因子NEUROD1、NEUROD2和ATH2在人类胎盘妊娠早期发育过程中表达。我们通过瞬时转染后使用实时定量逆转录聚合酶链反应（RT-PCR）进行绝对定量来测量内源性基因活性的变化，从而确定了这些因子在滋养层细胞中的反式激活潜力。在这些实验中，发现NEUROD1在滋养层细胞中瞬时反式激活NEUROD2。使用荧光素酶构建体的启动子截短实验表明，NEUROD2启动子中存在两个结构域，在转染NeuroD1后其活性增加。这些NeuroD1反应性结构域中的每一个都包含一个E-box序列。NEUROD2反式激活数据与NEUROD1和NEUROD2的空间表达模式相符，因为它们在内皮滋养层细胞中表达。这种表达模式以及目前的反式激活结果可能表明在人类胎盘妊娠早期发育过程中存在NEUROD分化级联反应。