人多发性骨髓瘤细胞系CZ-1的建立及其生物学特性

Establishment and biological characteristics of human multiple myeloma cell line CZ-1.

作者信息

Hou Jian, Lin Fa-ying, Zhang Bo, Zhang Ling-zhen, Ding Si-qi

机构信息

Department of Hematology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.

出版信息

Chin Med J (Engl). 2004 Jan;117(1):115-9.

PMID:14733786

Abstract

BACKGROUND

There were only 3 multiple myeloma (MM) cell lines established in China. In this study, we succeeded in establishing a novel MM cell line and analyzed its biological characteristics.

METHODS

Mononuclear cells isolated from the peripheral blood (PB) and bone marrow (BM) of a patient with advanced MM (lambda light chain type) were cultured in medium. Cell morphology was analyzed by Wright-Giemsa-staining and cytochemical staining, immunophenotyping by flow cytometry and cytogenetic analysis by chromosome RHG-banding technique. Quantitative fluorescent polymerase chain reaction (PCR) was used to detect Epstein - Barr virus (EBV) DNA.

RESULTS

The established cell line could survive and proliferate in the presence of feeder cells or conditioned medium. The cells secreted lambda light chain and were negative for EBV. The Wright-Giemsa-staining showed typical plasmablast or plasma cell morphology. The cytochemical staining of the cells showed the following reactivity patterns: positive for acid phosphatase, negative for myeloperoxidase. The immunoprofile of the cells was concordant with that of MM cells: positive for CD10, CD28, CD38, CD138, CD56, CD49d, CD44, CD54 and CD58, negative for CD19, CD40, CD95, CD95L, CD34, CD2 and CD5. The cytogenetic analysis showed complex chromosome abnormality of i (1q+), 8q+, 13q+, i (17q), i (18q) and +M. There was no difference in morphology, immunophenotype and cytogenetics between cells from PB and BM.

CONCLUSIONS

An MM cell line secreting lambda light chain named CZ-1 was established. The cells from both PB and BM have the same biological characteristics.

摘要

背景

中国仅建立了3种多发性骨髓瘤（MM）细胞系。在本研究中，我们成功建立了一种新型MM细胞系并分析了其生物学特性。

方法

从一名晚期MM（λ轻链型）患者的外周血（PB）和骨髓（BM）中分离出的单个核细胞在培养基中培养。通过瑞氏-吉姆萨染色和细胞化学染色分析细胞形态，通过流式细胞术进行免疫表型分析，通过染色体RHG显带技术进行细胞遗传学分析。采用定量荧光聚合酶链反应（PCR）检测爱泼斯坦-巴尔病毒（EBV）DNA。

结果

建立的细胞系在饲养细胞或条件培养基存在下能够存活和增殖。细胞分泌λ轻链且EBV呈阴性。瑞氏-吉姆萨染色显示典型的原始浆细胞或浆细胞形态。细胞化学染色显示以下反应模式：酸性磷酸酶阳性，髓过氧化物酶阴性。细胞的免疫表型与MM细胞一致：CD10、CD28、CD38、CD138、CD56、CD49d、CD44、CD54和CD58阳性，CD19、CD40、CD95、CD95L、CD34、CD2和CD5阴性。细胞遗传学分析显示存在复杂的染色体异常，即i（1q+）、8q+、13q+、i（17q）、i（18q）和+M。PB和BM来源的细胞在形态、免疫表型和细胞遗传学方面无差异。