Sun Yongjiang, Chan Roy Kum Wah, Tan Suat Hoon
Institute of Dermatology, Singapore National Skin Centre, 1 Mandalay Road, Singapore 308205, Singapore.
Virus Genes. 2004 Jan;28(1):129-33. doi: 10.1023/B:VIRU.0000012269.57481.f7.
In this study, the irntratypic variability of a tandem repeat locus within the DNA polymerase (pol) gene of human herpes simplex virus type 2 (HSV2) was uncovered. The locus contained variable numbers of tandem dodecanucleotide (5'-GAC GAG GAC GGG-3') repetitive units. Our result showed that approximately 95% of analyzed HSV2 clinical isolates and the current GenBank HSV2 strains contained two copies of the repetitive units. From genital herpes specimens, three new HSV2 strains, which respectively contained 1, 3, and 4 copies of the repetitive units, were identified. This variable number of tandem repeat (VNTR) locus is absent in HSV1, and thus it also contributes to the intertypic variability of HSV1 and HSV2. The intratypic variability of the locus may be useful for HSV2 strain genotyping and this application is discussed.
在本研究中，发现了人类单纯疱疹病毒2型（HSV2）DNA聚合酶（pol）基因内一个串联重复位点的型内变异性。该位点包含可变数量的串联十二核苷酸（5'-GAC GAG GAC GGG-3'）重复单元。我们的结果表明，约95%的分析HSV2临床分离株和当前GenBank中的HSV2菌株含有两份重复单元。从生殖器疱疹标本中，鉴定出三株新的HSV2菌株，它们分别含有1份、3份和4份重复单元。HSV1中不存在这种可变数量的串联重复（VNTR）位点，因此它也导致了HSV1和HSV2的型间变异性。该位点的型内变异性可能有助于HSV2菌株基因分型，并对此应用进行了讨论。
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