Chang Qing, Gong Shu-sheng, Ding Juan, Tang Ming, Hescheler Jürgen
Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2003 Aug;38(4):263-7.
To study the effect of linopirdine, a selective blocker of KCNQ channel family, on the whole-cell potassium currents in isolated outer hair cells (OHCs) and Deiters' cells of the cochlea from guinea pig and to probe the distribution of KCNQ channel family in OHC and Deiters' cells.
Using the patch clamp whole-cell recording, IK (tetraethylammonium-sensitive outward potassium currents) and IKa (inward potassium currents activated at negative potential) were measured before and after the administration of linopirdine in OHCs. Simultaneously, IK (the outward rectifier potassium current) was recorded in Deiters' cells.
IK was partly reduced after the administration of linopirdine in OHCs, moreover, IKa was totally inhibited. However, IK in Deiter's cells was not decreased.
KCNQ channel family could only involve in constructing the variant potassium channels in outer hair cells, but not in Deiters' cells.
研究KCNQ通道家族的选择性阻滞剂利诺吡啶对豚鼠耳蜗孤立外毛细胞（OHCs）和Deiters细胞全细胞钾电流的影响，并探究KCNQ通道家族在OHCs和Deiters细胞中的分布。
采用膜片钳全细胞记录法，在给予利诺吡啶前后测量OHCs中的IK（四乙铵敏感外向钾电流）和IKa（负电位激活的内向钾电流）。同时，记录Deiters细胞中的IK（外向整流钾电流）。
在OHCs中给予利诺吡啶后，IK部分降低，此外，IKa完全被抑制。然而，Deiters细胞中的IK未降低。
KCNQ通道家族仅参与构建外毛细胞中的可变钾通道，而不参与Deiters细胞中的钾通道构建。
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