Kruszewska Hanna, Misicka Aleksandra, Chmielowiec Urszula
National Institute of Public Health, 30/34 Chelmska, 00725, Warszawa, Poland.
Farmaco. 2004 Jan;59(1):13-20. doi: 10.1016/j.farmac.2003.10.002.
Thirty-two different microorganisms were examined in order to check their ability to degrade an exogenous DNA. Bacteria from species: Stenotrophomonas maltophilia, Brevundimonas diminuta, Bacillus subtilis, Mycobacterium butyricum and fungus Fusarium moniliforme were capable to degrade DNA to nucleic bases or their derivatives. Degradation of DNA by S. maltophilia resulted in formation of free bases, such as hypoxanthine, thymine, uracil and xanthine. The optimum concentration of DNA seemed to be 3 mg ml(-1). The mode of degradation of DNA nucleotides depended on the type of nucleotide and its concentration, but nucleic bases or their derivatives were always formed at the end of the reaction process.
为检测32种不同微生物降解外源DNA的能力，对嗜麦芽窄食单胞菌、缺陷短波单胞菌、枯草芽孢杆菌、丁酸分枝杆菌等细菌以及串珠镰刀菌进行了研究。结果发现，这些微生物能够将DNA降解为核酸碱基或其衍生物。嗜麦芽窄食单胞菌降解DNA后会形成游离碱基，如次黄嘌呤、胸腺嘧啶、尿嘧啶和黄嘌呤。DNA的最佳浓度似乎为3 mg ml(-1)。DNA核苷酸的降解模式取决于核苷酸的类型及其浓度，但在反应过程结束时总会形成核酸碱基或其衍生物。
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