B 型慢性淋巴细胞白血病中醛缩酶活性的细胞内和血清水平

Intracellular and serum levels of aldolase activity in B chronic lymphocytic leukemia.

作者信息

Musolino C, Alonci A, Allegra A, Di Cesare E, Orlando A, Grosso P, Buda G, Squadrito G

机构信息

Institute of Internal Medicine, Messina University, Italy.

出版信息

Int J Hematol. 1992 Dec;56(3):213-7.

PMID:1477333

Abstract

Intracellular and serum activities of aldolase (ALS) were biochemically determined in lymphocyte subpopulations from normal subjects and patients with B-chronic lymphocytic leukemia (B-CLL). Aldolase activity was significantly lower in T cells of CLL than in normal T cells (2.9 +/- 1.5 vs. 4.7 +/- 2.1 Sigma Units (SU)/6 x 10(6) cells, p < 0.05). The aldolase activity also was significantly (p < 0.001) lower (3.1 +/- 1.9 SU/6 x 10(6) cells) in CLL B lymphocytes than in normal B lymphocytes (18.1 +/- 6.5 SU/6 x 10(6) cells). Moreover, the serum levels of ALS in all patients with B-CLL were higher than that in normal subjects (8.1 +/- 5.8 vs. 2.2 +/- 0.8 SU/ml, p < 0.02). Our findings demonstrate that T lymphocytes from patients with B-CLL display enzyme activity different from that of normal T cells. This may reflect the abnormal maturity of the residual T cell population in B-CLL.

摘要

对正常受试者和B细胞慢性淋巴细胞白血病（B-CLL）患者淋巴细胞亚群中的醛缩酶（ALS）进行细胞内和血清活性的生化测定。CLL患者T细胞中的醛缩酶活性显著低于正常T细胞（2.9±1.5对4.7±2.1西格玛单位（SU）/6×10⁶个细胞，p<0.05）。CLL B淋巴细胞中的醛缩酶活性（3.1±1.9 SU/6×10⁶个细胞）也显著低于正常B淋巴细胞（18.1±6.5 SU/6×10⁶个细胞）（p<0.001）。此外，所有B-CLL患者的血清ALS水平均高于正常受试者（8.1±5.8对2.2±0.8 SU/ml，p<0.02）。我们的研究结果表明，B-CLL患者的T淋巴细胞表现出与正常T细胞不同的酶活性。这可能反映了B-CLL中残余T细胞群体的异常成熟。