SmD1是剪接前导RNA生物合成所必需的。
SmD1 is required for spliced leader RNA biogenesis.
作者信息
Zeiner Gusti M, Foldynová Silvie, Sturm Nancy R, Lukes Julius, Campbell David A
机构信息
Department of Microbiology, Immunology & Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095-1489, USA.
出版信息
Eukaryot Cell. 2004 Feb;3(1):241-4. doi: 10.1128/EC.3.1.241-244.2004.
Abstract
The Sm-binding site of the kinetoplastid spliced leader RNA has been implicated in accurate spliced leader RNA maturation and trans-splicing competence. In Trypanosoma brucei, RNA interference-mediated knockdown of SmD1 caused defects in spliced leader RNA maturation, displaying aberrant 3'-end formation, partial formation of cap 4, and overaccumulation in the cytoplasm; U28 pseudouridylation was unaffected.
摘要
动基体剪接前导RNA的Sm结合位点与精确的剪接前导RNA成熟和反式剪接能力有关。在布氏锥虫中，RNA干扰介导的SmD1敲低导致剪接前导RNA成熟缺陷，表现为异常的3'末端形成、帽4的部分形成以及在细胞质中的过度积累；U28假尿苷化不受影响。
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