Wu N, Wang T, Hartwick R A, Huie C W
Department of Chemistry, State University of New York, Binghamton 13902-6000.
J Chromatogr. 1992 Nov 6;582(1-2):77-85. doi: 10.1016/0378-4347(92)80305-a.
Four major bilirubin species in serum were separated by micellar electrokinetic chromatography with 25 mM sodium dodecyl sulfate (SDS) and 20 mM sodium tetraborate-boric acid buffer at pH 8.5. Due to the solubilization of the serum proteins by the SDS micelles, serum samples were injected directly into a 50 cm x 75 microns I.D. fused-silica capillary and complete separation of the four bilirubin species was accomplished within ca. 10 min without extensive sample pretreatment. Detection was performed by absorbance at 450 nm and average limit of detection was in the 6.0 microM concentration range. The usefulness of this method was demonstrated for the separation and detection of a number of bilirubin species present in pathological human serum samples.
采用胶束电动色谱法，以25 mM十二烷基硫酸钠（SDS）和20 mM四硼酸钠 - 硼酸缓冲液（pH 8.5）分离血清中的四种主要胆红素。由于SDS胶束可溶解血清蛋白，血清样品可直接注入内径为75微米、长度为50厘米的熔融石英毛细管中，无需进行大量样品预处理，约10分钟内即可完成四种胆红素的完全分离。通过在450 nm处的吸光度进行检测，平均检测限在6.0 microM浓度范围内。该方法在分离和检测病理性人血清样品中存在的多种胆红素方面得到了验证。
