大肠杆菌对碳二亚胺的抗性突变体：通过在葡萄糖或甘油上生长来抑制对二环己基碳二亚胺的抗性
Carbodiimide-resistant mutant of Escherichia coli: suppression of resistance to dicyclohexylcarbodiimide by growth on glucose or glycerol.
作者信息
Fillingame R H, Wopat A E
出版信息
J Bacteriol. 1978 May;134(2):687-9. doi: 10.1128/jb.134.2.687-689.1978.
Abstract
We have previously reported on the isolation of a mutant strain of Escherichia coli, RF-7, that has a dicyclohexylcarbodiimide (DCCD)-resistant, membrane-associated adenosine triphosphatase (ATPase) activity (R. H. Fillingame, J. Bacteriol. 124:870--883, 1975). We report here that the DCCD resistance of the ATPase of this mutant varies significantly, depending upon the carbon source used for growth. When strain RF-7 was grown aerobically on either glycerol or glucose or anaerobically on glucose rather than on a combination of succinate, acetate, and malate, ATPase activity was more sensitive to inhibition by DCCD because the carbodiimide-reactive proteolipid reacted more readily with DCCD.
摘要
我们之前报道过大肠杆菌突变株RF-7的分离，该菌株具有抗二环己基碳二亚胺（DCCD）的、与膜相关的腺苷三磷酸酶（ATPase）活性（R. H. 菲林盖姆，《细菌学杂志》124:870 - 883，1975年）。我们在此报告，该突变体ATPase的DCCD抗性有显著差异，这取决于用于生长的碳源。当菌株RF-7在甘油或葡萄糖上需氧生长，或在葡萄糖上厌氧生长，而非在琥珀酸盐、乙酸盐和苹果酸盐的组合上生长时，ATPase活性对DCCD抑制更敏感，因为碳二亚胺反应性蛋白脂质与DCCD反应更易发生。
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