Castro F, Viedma P, Cotorás D
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago.
Microbiologia. 1992 Nov;8(2):94-105.
The effect of different parameters on the adsorption of metal ions by Rhizopus oligosporus has been studied. The uranium sorption by dried biomass was rapid, reaching in 5 min around 95.% of the binding capacity. The uranium-binding capacity of the culture showed an inverse relation to the growth kinetic. The relationship between sorption and equilibrium concentration was similar to an adsorption isotherm. Using the Langmuir model, a maximum sorption capacity of 0.52 mmoles uranium/g dry biomass and an affinity constant of 101 l/mmol uranium at pH 4.15 were determined. The best capacity of the biomass to bind ions (UO2(2+), Cu2+, Cd2+ and Zn2+) was best at pH 4.5-5.5. By using hydrochloric acid as eluant a 18% uranium removal of the biomass-bound ions was obtained at pH 1.0. The presence of other cations inhibited uranium-binding in the following order: Cu2+ > Cd2+ > Zn2+ > Mg2+ > Ca2+.
研究了不同参数对少孢根霉吸附金属离子的影响。干燥生物质对铀的吸附很快，5分钟内达到结合容量的95%左右。培养物的铀结合能力与生长动力学呈反比关系。吸附与平衡浓度之间的关系类似于吸附等温线。使用朗缪尔模型，在pH 4.15时，确定最大吸附容量为0.52毫摩尔铀/克干生物质，亲和常数为101升/毫摩尔铀。生物质结合离子（UO2(2+)、Cu2+、Cd2+和Zn2+）的最佳能力在pH 4.5 - 5.5时最佳。以盐酸为洗脱剂，在pH 1.0时可从生物质结合的离子中去除18%的铀。其他阳离子的存在按以下顺序抑制铀结合：Cu2+ > Cd2+ > Zn2+ > Mg2+ > Ca2+。
