M'Fouara J C, Bouziane M N, Prost J, Belleville J
Université de Bourgogne, Faculté des Sciences Mirande, Dijon.
C R Seances Soc Biol Fil. 1992;186(3):263-77.
Requirements in polyunsaturated fatty acids (PUFA) of series n-3 and n-6 may be amplified and their metabolism, transport, and utilization may be impaired in the long term, by protein depletion. The aim of this study was to evaluate, in young rats, malondialdehyde (MDA) production and erythrocyte membrane antioxidative defense, when they were fed balanced (20% casein) or depleted (2% casein) protein diet associated with various oils (sunflower, soybean, coconut or salmon). Over a short period (28 days), eight groups of 10 male Wistar rats were fed eight different diets: TOC (20% casein + 5% sunflower oil), TOd (2% casein + 5% soybean oil), SOC (20% casein + 5% soybean oil), SOd (2% casein + 5% soybean oil), COC (20% casein + 5% coconut oil), COd (2% casein + 5% coconut oil), SAC (20% casein + 5% salmon oil), SAd (2% casein + 5% salmon oil). Blood was removed, MDA was assessed in plasma (reaction with thiobarbituric acid). Washed erythrocytes were subjected to organic free radical generator (Kit KGRL 400 Spiral R.D., Couternon, 21560 France). The haemoglobin released was analysed by spectrophotometry. The total anti-radical defense status was expressed as the length of time to reach 50% hemolysis (T 50% in min). Plasma of deficient groups (2% casein) exhibited low concentrations of protein, particularly with coconut and salmon oils; phospholipid and total cholesterol, excepted with diet containing coconut oil; triacylglycerol; and VLDL. Malondialdehyde. In groups fed balanced protein diets, the lowest values were obtained with salmon and coconut oils. MDA contents of groups TOd, COd and SAd were higher than those of their respective control groups, but significantly only in group COd. Antiradical defense status. Total anti-radical defence status in erythrocytes was not modified in the short term by balanced or depleted protein diets which ever oil was used, despite deep changes in fatty acid composition of membrane phospholipids. In particular, phospholipid contents in eicosapentaenoic, docosahexaenoic acids were greatly enhanced by the consumption of salmon oil compared to the values obtained with coconut oil.
长期的蛋白质缺乏可能会增加对n-3和n-6系列多不饱和脂肪酸（PUFA）的需求，并且可能损害它们的代谢、运输和利用。本研究的目的是评估幼鼠在喂食与各种油类（向日葵油、大豆油、椰子油或三文鱼油）相关的平衡（20%酪蛋白）或缺乏（2%酪蛋白）蛋白质饮食时丙二醛（MDA）的产生和红细胞膜抗氧化防御能力。在短时间内（28天），将八组每组10只雄性Wistar大鼠喂食八种不同的饮食：TOC（20%酪蛋白 + 5%向日葵油）、TOd（2%酪蛋白 + 5%大豆油）、SOC（20%酪蛋白 + 5%大豆油）、SOd（2%酪蛋白 + 5%大豆油）、COC（20%酪蛋白 + 5%椰子油）、COd（2%酪蛋白 + 5%椰子油）、SAC（20%酪蛋白 + 5%三文鱼油）、SAd（2%酪蛋白 + 5%三文鱼油）。采集血液，评估血浆中的MDA（与硫代巴比妥酸反应）。将洗涤后的红细胞置于有机自由基发生器（Kit KGRL 400 Spiral R.D.，法国库特农，邮编21560）中。通过分光光度法分析释放的血红蛋白。总抗自由基防御状态表示为达到50%溶血的时间长度（以分钟计的T 50%）。缺乏蛋白质组（2%酪蛋白）的血浆中蛋白质浓度较低，特别是与椰子油和三文鱼油搭配时；磷脂和总胆固醇（含椰子油的饮食除外）；三酰甘油；以及极低密度脂蛋白。丙二醛。在喂食平衡蛋白质饮食的组中，三文鱼油和椰子油组的值最低。TOd、COd和SAd组的MDA含量高于各自的对照组，但仅COd组差异显著。抗自由基防御状态。无论使用何种油类，平衡或缺乏蛋白质饮食在短期内均未改变红细胞中的总抗自由基防御状态，尽管膜磷脂的脂肪酸组成发生了深刻变化。特别是，与椰子油相比，食用三文鱼油可使二十碳五烯酸、二十二碳六烯酸中的磷脂含量大幅增加。
