Borovsky D, Carlson D A
IFAS-University of Florida, Florida Medical Entomology Laboratory, Vero Beach 32962.
J Med Entomol. 1992 Mar;29(2):318-24. doi: 10.1093/jmedent/29.2.318.
A technique has been developed to study JH III biosynthesis in vitro by removing the head and thorax of Aedes aegypti (L.) and exposing the corpora allata (CA). Exposed CA were incubated with [12-3H]methyl farnesoate, and the newly synthesized JH III and JH III metabolites were followed using C18 reversed-phase, high-performance liquid chromatography (HPLC) and preparative gas chromatography. The rate of synthesis of [12-3H]JH III by exposed CA from newly emerged adult Ae. aegypti was 23 fmol/CA/h. The rate of synthesis of [12-3H]JH III decreased 4-fold 3 d after adult eclosion and increased to 23 fmol/CA/h 5 h after the blood meal. Exposed CA of blood-fed and sugar-fed Ae. aegypti were also incubated with L-[methyl-3H]methionine, and the rate of synthesis of JH III was studied. The rate of JH III biosynthesis increased immediately after the blood meal as was found with [12-3H]methyl farnesoate. The potential application of methyl farnesoate in monitoring the de novo synthesis of JH III in mosquitoes in vitro and in vivo is discussed.
已开发出一种技术，通过切除埃及伊蚊（L.）的头部和胸部并暴露咽侧体（CA）来体外研究保幼激素III（JH III）的生物合成。将暴露的咽侧体与[12-³H]法尼酸甲酯一起孵育，并使用C18反相高效液相色谱（HPLC）和制备气相色谱追踪新合成的JH III和JH III代谢物。新羽化的成年埃及伊蚊暴露的咽侧体合成[12-³H]JH III的速率为23 fmol/CA/小时。羽化后3天，[12-³H]JH III的合成速率下降了4倍，而在进食血液后5小时增加到23 fmol/CA/小时。还将进食血液和进食糖水的埃及伊蚊暴露的咽侧体与L-[甲基-³H]甲硫氨酸一起孵育，并研究JH III的合成速率。正如用[12-³H]法尼酸甲酯所发现的那样，进食血液后JH III生物合成的速率立即增加。讨论了法尼酸甲酯在体外和体内监测蚊子中JH III从头合成的潜在应用。
