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超声处理后美洲大蠊中枢神经系统的ATP酶Mg++及Mg++ Na+ K+活性的改变(作者译)

[Alteration of the ATP-ase Mg++ and Mg++ Na+ K+ activities of the central nervous system of Periplaneta americana L. after sonification. (author's transl].

作者信息

Holzhacker E L, Fell D, Giannotti O

出版信息

Arq Inst Biol (Sao Paulo). 1977 Oct-Dec;44(4):215-24.

PMID:149528
Abstract

A prolonged ultrasonic homogenization of a Periplaneta americana L. suspension of ganglia in a buffer solution pH 7,6 when compared with a Potter Elvehjem homogenization shows a marked reduction of Mg++ and (Mg++ + Na+ + K+) ATPase activities. Mg++ ATPase was not modified while the (Mg++ + Na+ + K+) ATPase was completely inhibited after 30 second ultrasonic homogenization. This probably results from an enzyme structure alteration caused by the ultrasonic treatment.

摘要

与波特-埃尔维耶姆匀浆法相比,在pH 7.6的缓冲溶液中对美洲大蠊神经节悬浮液进行长时间超声匀浆,结果显示Mg++和(Mg++ + Na+ + K+)ATP酶活性显著降低。Mg++ ATP酶未发生改变,而在超声匀浆30秒后,(Mg++ + Na+ + K+)ATP酶被完全抑制。这可能是由于超声处理导致酶结构改变所致。

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