携带氨基和硫醇官能团的2'-脱氧胞苷：由Vent（外切）聚合酶进行的合成与掺入

2'-deoxycytidines carrying amino and thiol functionality: synthesis and incorporation by Vent (exo-) polymerase.

作者信息

Roychowdhury Abhijit, Illangkoon Heshan, Hendrickson Cynthia L, Benner Steven A

机构信息

Department of Chemistry, University of Florida, P.O. Box 117200, Gainesville, Florida 32611, USA.

出版信息

Org Lett. 2004 Feb 19;6(4):489-92. doi: 10.1021/ol0360290.

DOI:10.1021/ol0360290

PMID:14961605

Abstract

[reaction: see text] The synthesis of 2'-deoxycytidine nucleosides bearing amino and thiol groups appended to the 5-position of the nucleobase via a butynyl linker is described. The corresponding triphosphates were then synthesized from the nucleoside and incorporated into oligonucleotides by Vent (exo(-)) DNA polymerase. The ability of Vent (exo(-)) polymerase to amplify oligonucleotides containing these functionalized cytidine derivatives in a polymerase chain reaction (PCR) was demonstrated for the amino-functionalized derivative.

摘要

[反应：见正文] 描述了通过丁炔基连接子在核苷酸碱基的5位连接有氨基和硫醇基的2'-脱氧胞苷核苷的合成。然后从核苷合成相应的三磷酸酯，并通过Vent（外切（-））DNA聚合酶掺入寡核苷酸中。对于氨基官能化衍生物，证明了Vent（外切（-））聚合酶在聚合酶链反应（PCR）中扩增含有这些官能化胞苷衍生物的寡核苷酸的能力。